Nachweis der Penetrationstiefe von BioRoot - Sealer in Dentintubuli unter Anwendung verschiedener Obturationsmethoden

Endodontology deals with teaching, treatment and prevention of diseases of the endodont (pulp-dentin complex) and periapical tissues. The aim is to achieve long-term preservation of the stomatognathic system, contributed to by sufficient root canal fillings. More precisely, sufficient root canal filling depends on several factors. One is sufficient sealer penetration into the freely exposed dentinal tubules after uniform canal preparation. In 2016, a new tricalcium-based sealer, BioRoot RCS, from Septodont was introduced to the dental trade. On the one hand, the biocompatible sealant and, on the other hand, the alkaline pH value of >11 generated by the release of calcium ions should enable sufficient completion of the root canal filling. Aim The purpose of this study was to demonstrate the penetration depth of BioRoot RCS in different obturation methods and always in comparison to a reference group consisting of AH Plus. 6.2 Materials and Methods A total of 60 human single-rooted teeth were divided into four study groups. All teeth were cleaned and prepared with the F360 filing system. Root canal irrigation was performed using a standardized irrigation protocol consisting of NaOCl 5.25 % and EDTA 15 %. The four study groups included the reference group (AH Plus) LC (lateral condensation) filled, the BioRoot RCS LC filled group, an SC filled group with BioRoot RCS, and a group filled with Biodentine and BioRoot. The fluorescent dyes rhodamine B isotiocyanate and fluorescein sodium were added tot he sealers prior to use. After four weeks of curing of the sealers in the root canals, the samples were sawed and examined under the confocal laser scanning microscope.49 Results The reference group with AH Plus showed significantly less penetration depths of 79 µm compared to BioRoot RCS with significantly increased 159 – 164 µm penetration depth in the apical and middle sextions oft he root canals. In the middle root region, the study groups differentiated, with BioRoot RCS LC penetrating significantly deeper at 329 µm to AH Plus LC 230 µm in a direct comparison of the filling technique. The coronal penetration of LC with BioRoot RCS 237 µm was inferior to LC with AH Plus by 295 µm. Nevertheless, the SC technique achieved similar results to LC with AH Plus at 293 µm – 295 µm. The median values of group 4 to be investigated with Biodentine was always on par with the LC technique with AH Plus. (see chart 2) Conclusion Within the limits of this in vitro study, it can be concluded that BioRoot RCS penetrates deeper into dentinal tubules than the comparison groups with AH Plus. BioRoot RCS penetrates apically deeper than the reference group in both filling groups. In the medial region, lateral condensation has higher penetration depths than AH Plus. Coronally, however, all study groups show approximately similar values.