Knockdown of CRPV E6 and E7 Oncogenes Using Lipopolyplexes as Transfecting Reagents

The VX2 carcinoma model from the New Zealand white rabbits serves as a suitable model system for human head and neck squamous cell carcinoma. Both cancer models share the common papilloma virus. HPV and CRPV both possess the oncogenes E2, E6 and E7. This thesis deals with one of the most vital field...

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Bibliographic Details
Main Author: Ali, Uzma
Contributors: Bakowsky, Udo (Prof. Dr) (Thesis advisor)
Format: Dissertation
Language:English
Published: Philipps-Universität Marburg 2021
Subjects:
Online Access:PDF Full Text
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Summary:The VX2 carcinoma model from the New Zealand white rabbits serves as a suitable model system for human head and neck squamous cell carcinoma. Both cancer models share the common papilloma virus. HPV and CRPV both possess the oncogenes E2, E6 and E7. This thesis deals with one of the most vital fields of molecular targeting therapy, the siRNA induced gene silencing. An extensive literature review-based concept of parallel therapeutic options for HPV and CRPV induced carcinomas using siRNA has been addressed. Transfection of siRNA into cancer cells is very important for the successful RNAi mediated knockdown of oncogenes, therefore the pros and cons of using lipopolyplexes for transfecting VX2 cells and a model COS-7 cell line have been analysed. The introduction part of this thesis comprises of an overview of
Physical Description:129 Pages
DOI:https://doi.org/10.17192/z2021.0053