The mechanism of pRNA-mediated release of RNA polymerase from Bacillus subtilis 6S-1 RNA
Adaptation of the transcriptome to nutrient limitation and resupply is a fundamental process in bacteria, particularly in natural habitats. Bacterial 6S RNA, an ubiquitous and growth phasedependent regulator of transcription, binds to RNA polymerase (RNAP) and inhibits transcription during stati...
Đã lưu trong:
| Tác giả chính: | |
|---|---|
| Tác giả khác: | |
| Định dạng: | Dissertation |
| Ngôn ngữ: | Tiếng Anh |
| Được phát hành: |
Philipps-Universität Marburg
2010
|
| Những chủ đề: | |
| Truy cập trực tuyến: | Bài toàn văn PDF |
| Các nhãn: |
Không có thẻ, Là người đầu tiên thẻ bản ghi này!
|
| Tóm tắt: | Adaptation of the transcriptome to nutrient limitation and resupply
is a fundamental process in bacteria, particularly in natural
habitats. Bacterial 6S RNA, an ubiquitous and growth phasedependent
regulator of transcription, binds to RNA polymerase
(RNAP) and inhibits transcription during stationary growth.
Upon nutrient resupply, RNAP acts as an RNA-dependent RNA
polymerase by transcribing large amounts of short RNAs (pRNAs)
from 6S RNA as template, leading to dissociation of 6S RNARNAP
complexes. Whereas the majority of bacteria express a
single 6S RNA species, Bacillus subtilis encodes two 6S RNAs
(6S-1 and 6S-2) of similar secondary structure, but with different
expression profiles.
In this work, we investigated the two 6S RNAs of B. subtilis,
focusing on pRNA synthesis and its role for the function of 6S
RNA. Concurrently, we identified pRNA transcription from 6S-1
RNA in vivo using high-troughput sequencing techniques and we
developed a novel Northern hybridization protocol for detection
of pRNAs in bacterial total cellular extracts.
Our results show that the release of RNAP from 6S-1 RNA,
the functional homolog of the well investigated E. coli 6S RNA,
is regulated by stable pRNA binding. Additionally, we found
structural changes of 6S-1 RNA, induced by differences in pRNA
length in different growth phases. This specific structural change
of 6S RNA seems to be conserved among bacteria. Furthermore,
we are able to show that the two processes of RNAP release and
6S-1 RNA degradation are coupled in vivo. Taken together, our
results expand the current understanding of 6S RNA function
and provide insight into the mechanism of RNAP release from
6S RNA in bacteria.
iii |
|---|---|
| Mô tả vật lý: | 201 Seiten |
| DOI: | 10.17192/z2010.0758 |