Untersuchung zur Serodiagnostik von Cox B – Viren - Ein Methodenvergleich

Enterovirus-or CBV infections can do a huge number of clinical symptoms hervor-rufen and as a rule at most a suspicion diagnosis can be put. To avoid unnecessary treatments and investigations, it can be necessary to have to put a precise diagnosis. This counts in particular to serious illness courses or illnesses with newborn children with which CBV infections show not seldom a fulminating course. Two basic procedures are available for the diagnostics, namely the direct proof of the viruses, possibly by the Anzüchtung of the viruses and the anschließen-de specification, or the indirect proof by regulation of antibodies. In our study were applied, as indirect proof procedures, the Neutralisatinstest (NT) and the ELISA test with the Seren by a total of 523 patients with whom the suspicion diagnosis of a Myokarditis had been put. The purpose of the investigation was to compare both test procedures with each other to point, to what extent the ELISA test the NT to substituted ones can do. Besides, different NT-Grenztiter were laid as presumably positive findings as a basis. Further tried should become to determine the interest of the presumably acute illnesses. On this occasion, age distribution and gender distribution as well as the frequency of the single CBV types berücksich-tigt should also become. To grasp patients with serologischen tips to a fresh or active infection possibly only, a Grenztiter of 80 was fixed for the NT. Besides, an interest of positive findings of 64.0 percent was found. In the ELISA test an interest of positive findings of 33.8 percent was found (28.5% there were IgG positively, besides). Most often AK were found against Cox-B4 (34.9%), followed by Cox-B2 (22.1%), Cox-B3 (20.5%), Cox B 5 (11.5%) and Cox-B1 (9.4%). A dependence on single old groups did not arise with the NT. With the ELISA test the number positive Seren with increasing age decreased. Differences specific for gender could not be shown with the NT. With the ELISA test women had significantly more often positive findings as men (40.3 versus 30.2%; p <0.05). Under acceptance of the results of the NT as a "true" size (positive result = active lich positive findings) the ELISA test showed only one low sensitivity which swayed as a function of NT limit value between 33.9 and 38.2 percent. An increase of the sensitivity with rising NT limit value could not be shown. The specificity swayed between 46.2 and 58.1 percent and did not likewise correlate with the NT limit value. One was found out that the ELISA test is suitable only partly to prove acute or tramped CBV infections. A Korrela-tion between the ELISA test and the NT was not. The ELISA test seems not suitable on account of the results, around the NT substituted to.