Let it glow: genetically encoded fluorescent reporters in Plasmodium
The use of fluorescent proteins (FPs) in Plasmodium parasites has been key to understand the biology of this obligate intracellular protozoon. FPs like the green fluorescent protein (GFP) enabled to explore protein localization, promoter activity as well as dynamic processes like protein export an...
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| Huvudskapare: | , , , |
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| Materialtyp: | Artikel |
| Språk: | engelska |
| Publicerad: |
Philipps-Universität Marburg
2024
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| Länkar: | PDF-fulltext |
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| Sammanfattning: | The use of fluorescent proteins (FPs) in Plasmodium parasites has been key to understand the biology of this
obligate intracellular protozoon. FPs like the green fluorescent protein (GFP) enabled to explore protein localization,
promoter activity as well as dynamic processes like protein export and endocytosis. Furthermore, FP biosensors
have provided detailed information on physiological parameters at the subcellular level, and fluorescent reporter
lines greatly extended the malariology toolbox. Still, in order to achieve optimal results, it is crucial to know exactly
the properties of the FP of choice and the genetic scenario in which it will be used. This review highlights advantages
and disadvantages of available landing sites and promoters that have been successfully applied for the ectopic
expression of FPs in Plasmodium berghei and Plasmodium falciparum. Furthermore, the properties of newly developed
FPs beyond DsRed and EGFP, in the visualization of cells and cellular structures as well as in the sensing of small
molecules are discussed |
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| Beskrivning: | Gefördert durch den Open-Access-Publikationsfonds der UB Marburg. |
| DOI: | 10.1186/s12936-024-04936-9 |