Publikationsserver
The ratio of cytochrome c oxidase subunit 4 isoform 4I1 and 4I2 mRNA is changed in permanent atrial fibrillation
Aims: The conditions of hypoxia are suggested to induce permanent atrial fibrillation (AF). The regulation of COX4I2 and COX4I1 depends on oxygen availability in tissues. A role of COX4I2 in the myocardium of AF patients is supposed for pathogenesis of AF and subsequent alterations in the electron...
Spremljeno u:
| Glavni autori: | , , , , , , , , , , |
|---|---|
| Format: | Članak |
| Jezik: | engleski |
| Izdano: |
Philipps-Universität Marburg
2023
|
| Teme: | |
| Online pristup: | PDF cijeli tekst |
| Oznake: |
Dodaj oznaku
Bez oznaka, Budi prvi tko označuje ovaj zapis!
|
| Sažetak: | Aims: The conditions of hypoxia are suggested to induce permanent atrial fibrillation (AF). The regulation of COX4I2 and
COX4I1 depends on oxygen availability in tissues. A role of COX4I2 in the myocardium of AF patients is supposed for pathogenesis
of AF and subsequent alterations in the electron transfer chain (ETC) under hypoxia.
Methods and results: In vitro, influence of hypoxia on HeLa 53 cells was studied and elevated parts of COX 4I2 were confirmed.
Myocardial biopsies were taken ex vivo from the patients’ Right Atria with SR (n = 31) and AF (n = 11), respectively.
RT- PCR for mRNA expresson, mitochondrial respiration by polarography and the protein content of cytochrome c oxidase
(CytOx) subunit 4I1 and CytOx subunit 4I2 by ELISA were studied. Clinical data were correlated to the findings of gene expressions
in parallel. Patients with permanent AF had a change in isoform 4I2/4I1 expression along with a decrease of isoform COX
4I1 expression. The 4I2/4I1 ratio of mRNA expression was increased from 0.630 to 1.058 in comparison. However, the protein
content of CytOx subunit 4 was much lower in the AF group, whereas the respiration/units enzyme activity in both groups
remained the same.
Conclusions: This study describes a possible molecular correlate for the development of AF. Due to the known functional significance
of COX 4I2, mitochondrial dysfunction can be assumed as a part of the pathogenesis of AF. |
|---|---|
| Opis predmeta: | Gefördert durch den Open-Access-Publikationsfonds der UB Marburg. |
| Digitalni identifikator objekta: | 10.1002/ehf2.14607 |