NMOSD IgG Impact Retinal Cells in Murine Retinal Explants

NMOSD IgG Impact Retinal Cells in Murine Retinal Explants

Neuromyelitis optica spectrum disorders (NMOSD) are chronic inflammatory diseases of the central nervous system, characterized by autoantibodies against aquaporin-4. The symptoms primarily involve severe optic neuritis and longitudinally extensive transverse myelitis. Although the disease progres...

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Bibliografische gegevens
Hoofdauteurs: Wolf, Hannah Nora, Ehinger, Veronika, Guempelein, Larissa, Banerjee, Pratiti, Kuempfel, Tania, Havla, Joachim, Pauly, Diana
Formaat: Artikel
Taal:Engels
Gepubliceerd in: Philipps-Universität Marburg 2023
Onderwerpen:
autoantibodies
Medizin
chemokine
mouse retinal explants
NMOSD
local
retina
complement
Müller cell
Medical sciences Medicine
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Samenvatting:Neuromyelitis optica spectrum disorders (NMOSD) are chronic inflammatory diseases of the central nervous system, characterized by autoantibodies against aquaporin-4. The symptoms primarily involve severe optic neuritis and longitudinally extensive transverse myelitis. Although the disease progression is typically relapse-dependent, recent studies revealed retinal neuroaxonal degeneration unrelated to relapse activity, potentially due to anti-aquaporin-4-positive antibodies interacting with retinal glial cells such as Müller cells. In this exploratory study, we analysed the response of mouse retinal explants to NMOSD immunoglobulins (IgG). Mouse retinal explants were treated with purified IgG from patient or control sera for one and three days. We characterized tissue response patterns through morphological changes, chemokine secretion, and complement expression. Mouse retinal explants exhibited a basic proinflammatory response ex vivo, modified by IgG addition. NMOSD IgG, unlike control IgG, increased gliosis and decreased chemokine release (CCL2, CCL3, CCL4, and CXCL-10). Complement component expression by retinal cells remained unaltered by either IgG fraction. We conclude that human NMOSD IgG can possibly bind in the mouse retina, altering the local cellular environment. This intraretinal stress may contribute to retinal degeneration independent of relapse activity in NMOSD, suggesting a primary retinopathy.
Beschrijving item:Gefördert durch den Open-Access-Publikationsfonds der UB Marburg.
DOI:10.3390/cimb45090463

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