Parietin cyclodextrin-inclusion complex as an effective formulation for bacterial photoinactivation
: Multidrug resistance in pathogenic bacteria has become a significant public health concern. As an alternative therapeutic option, antimicrobial photodynamic therapy (aPDT) can successfully eradicate antibiotic-resistant bacteria with a lower probability of developing resistance or systemic toxi...
সংরক্ষণ করুন:
প্রধান লেখক: | , , , , , , , , |
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বিন্যাস: | প্রবন্ধ |
ভাষা: | ইংরেজি |
প্রকাশিত: |
Philipps-Universität Marburg
2022
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বিষয়গুলি: | |
অনলাইন ব্যবহার করুন: | পিডিএফ এ সম্পূর্ন পাঠ |
ট্যাগগুলো: |
ট্যাগ যুক্ত করুন
কোনো ট্যাগ নেই, প্রথমজন হিসাবে ট্যাগ করুন!
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সংক্ষিপ্ত: | : Multidrug resistance in pathogenic bacteria has become a significant public health concern.
As an alternative therapeutic option, antimicrobial photodynamic therapy (aPDT) can successfully
eradicate antibiotic-resistant bacteria with a lower probability of developing resistance or systemic
toxicity commonly associated with the standard antibiotic treatment. Parietin (PTN), also termed
physcion, a natural anthraquinone, is a promising photosensitizer somewhat underrepresented in
aPDT because of its poor water solubility and potential to aggregate in the biological environment.
This study investigated whether the complexation of PTN with (2-hydroxypropyl)-β-cyclodextrin
(HP-β-CD) could increase its solubility, enhance its photophysical properties, and improve its phototoxicity against bacteria. At first, the solubilization behavior and complexation constant of the
PTN/HP-β-CD inclusion complexes were evaluated by the phase solubility method. Then, the
formation and physicochemical properties of PTN/HP-β-CD complexes were analyzed and confirmed in various ways. At the same time, the photodynamic activity was assessed by the uric acid
method. The blue light-mediated photodegradation of PTN in its free and complexed forms were
compared. Complexation of PTN increased the aqueous solubility 28-fold and the photostability
compared to free PTN. PTN/HP-β-CD complexes reduce the bacterial viability of Staphylococcus
saprophyticus and Escherichia coli by > 4.8 log and > 1.0 log after irradiation, respectively. Overall,
the low solubility, aggregation potential, and photoinstability of PTN were overcome by its complexation in HP-β-CD, potentially opening up new opportunities for treating infections caused by
multidrug-resistant bacteria. |
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উপাদানের বিবরণ: | Gefördert durch den Open-Access-Publikationsfonds der UB Marburg. |
দৈহিক বর্ননা: | 21 Seiten |
ডিওআই: | 10.3390/pharmaceutics14020357 |