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Flowzytometrische Differenzierung der Leukozytensubpopulationenim menschlichen Ejakulat
Nach WHO Richtlinien wird bei jeder Ejakulatanalyse die Zahl der seminalen Leukozyten bestimmt, da diese bei Überschreitung der Norm Hinweis auf eine Enzündung des Genitaltraktes geben können. In dieser Arbeit werden mehrere Antikörper, die der Erfassung und Differenzierung der Leukozyten sowie de...
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| Formato: | Dissertation |
| Lenguaje: | alemán |
| Publicado: |
Philipps-Universität Marburg
2005
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| Materias: | |
| Acceso en línea: | Texto Completo PDF |
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According to WHO guide lines seminal leucocytes are quantified in every semen analysis as they can be an indicator of genital tract inflammation. Ten monoclonal antibodies which serve to determine the differentiation and grade of activation of the leucocytes are tested on their qualification to be used in semen. The flowcytometric results of white blood cells in semen are compared to those of peripheral leucocytes. Only the pan leucocyte antibodies CD53 and CD45 as well as the marker for monocytes (CD14) are fit for differentiating white blood cells in semen. Staining of granulocytes is complicated by crossreaction of the antibodies with other semen particles. Seminal lymphocytes can’t be further differentiated into B- and T- cells because of low number. Differentiatin of leucocytes by flow cytometry is much more difficult in semen than in blood. This study shows that not all monoclonal antibodies against white blood cells are able to be used in semen.