Beiträge zur Herstellung von Hybridkatalysatoren am Modell der Carboanhydrase II

Die Dissertation beschreibt zwei unterschiedliche Ansätze zur Herstellung von Hybridkatalysatoren. Einerseits die erfolgreiche Durchführung einer Huisgen Cycloaddition in der aktiven Tasche der Carboanhydrase II. Als Edukte dienten ein kovalent gebundenes Azid und ein mithilfe eines Sulfonamids im a...

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Bibliographic Details
Main Author: Sandner, Nicola
Contributors: Koert, Ulrich (Prof. Dr.) (Thesis advisor)
Format: Doctoral Thesis
Published: Philipps-Universität Marburg 2015
Online Access:PDF Full Text
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The dissertation describes two different approaches to the synthesis of hybrid catalytic systems. On the one hand it was possible to conduct a Huisgen cycloaddition within the active site of the carbonic anhydrase II. The azide was bound covalently to the enzyme while the alkyne was anchored via a sulfonamide to the active site. The reaction could be monitored via crystal structural analysis and mass spectroscopic analysis. On the other hand the dissertation deals with contributions to the synthesis of a hydrogenation catalytic system consisting of a phosphine-rhodium-complex covalently bound to the carbonic anhydrase II. In the course of this work a water soluble phosphine-ligand could be synthesized with different reactive groups for covalent attachment to a cysteine.