Crosslinking studies of the bacterial RNase P holoenzyme and functional characterisation of the novel protein-only RNase P from Arabidopsis thaliana

Crosslinking studies of the bacterial RNase P holoenzyme and functional characterisation of the novel protein-only RNase P from Arabidopsis thaliana

Ribonuclease P (RNase P) is the ubiquitous processing enzyme that generates the mature 5'-termini of tRNA molecules. In Bacteria and Archaea the enzyme is a ribonucleoprotein, whereas in some Eukarya the RNAse P is exclusively proteinaceous. This work is focused on two topics: (i) crosslinking...

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Bibliografiska uppgifter
Huvudupphovsman: Pavlova, Liudmila Viktorovna
Övriga upphovsmän: Hartmann, Roland (Prof. Dr.) (BetreuerIn (Doktorarbeit))
Materialtyp: Dissertation
Språk:engelska
Publicerad: Philipps-Universität Marburg 2012
Ämnen:
Kinetics
Crosslinking
Proteinaceous RNase P
Catalytical mechanism
Endoribonuklease P
Naturwissenschaften
Natural sciences & mathematics
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Sammanfattning:Ribonuclease P (RNase P) is the ubiquitous processing enzyme that generates the mature 5'-termini of tRNA molecules. In Bacteria and Archaea the enzyme is a ribonucleoprotein, whereas in some Eukarya the RNAse P is exclusively proteinaceous. This work is focused on two topics: (i) crosslinking studies of bacterial RNase P holoenzyme utilizing novel 2’- ribooligonucleotides and (ii) functional investigations to the catalytical mechanism of recently discovered protein-only RNase P from Arabidopsis thaliana.
DOI:10.17192/z2012.0778

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