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Crosslinking studies of the bacterial RNase P holoenzyme and functional characterisation of the novel protein-only RNase P from Arabidopsis thaliana
Ribonuclease P (RNase P) is the ubiquitous processing enzyme that generates the mature 5'-termini of tRNA molecules. In Bacteria and Archaea the enzyme is a ribonucleoprotein, whereas in some Eukarya the RNAse P is exclusively proteinaceous. This work is focused on two topics: (i) crosslinking...
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| Formato: | Dissertation |
| Idioma: | inglês |
| Publicado em: |
Philipps-Universität Marburg
2012
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| Acesso em linha: | Texto integral em PDF |
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| Resumo: | Ribonuclease P (RNase P) is the ubiquitous processing enzyme that generates the mature 5'-termini of tRNA molecules. In Bacteria and Archaea the enzyme is a ribonucleoprotein, whereas in some Eukarya the RNAse P is exclusively proteinaceous. This work is focused on two topics: (i) crosslinking studies of bacterial RNase P holoenzyme utilizing novel 2’- ribooligonucleotides and (ii) functional investigations to the catalytical mechanism of recently discovered protein-only RNase P from Arabidopsis thaliana. |
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| DOI: | 10.17192/z2012.0778 |