Neue Ansätze zur Entwicklung eines Ganzzellbiosensors

Using surface plasmon resonance (SPR) different model applications were established. Different sugar surfaces were prepared and characterised by a lectin screening. The binding behaviour of such surfaces was examined by NCI-H125 and Lewis Lung cells. In order to determine mistletoe lectin in drugs for cancer therapy a mistletoe lectin assay was developed. The detection of recombinant mistletoe lectin I (MLI) was performed by two different monoclonal anti-MLI-antibodies. Another model application was the visualisation of the non-enzymatic degradation of layer thickness of polyethylene carbonate by superoxide radical anions using murine macrophages of cell line J774A.1. The rate of degradation could be influenced by the trigger factors Concanavalin A, LPS from Escherichia coli and Salmonella typhimurium. Gram negative bacteria as a trigger for macrophages were imitated by a bacterial membrane model. For this purpose liposomes were functionalised with lipopolysaccharide from Legionella pneumophila and Salmonella typhimurium and detected using specific antibodies. Another mode of triggering murine macrophages from cell line J774A.1 was performed by the use of LPS-coupled magnetic beads implemented on the LAPS (light-addressable potentiometric sensor) measurement platform. By utilisation of a permanent magnet a concentration gradient was produced in the test solution. The metabolic activity of macrophages could be measured as a change in pH-value.