In vitro-Untersuchung zur Erreger-eliminierenden Wirkung von Wundspüllösungen

The implementation of precise lavage combined with thoroughly executed debridement has an essential significance in wound management of acute care in traumatological patients in order to prevent wound healing disorders and potential infections. Miscellaneous antiseptic wound-cleansing means as well as sterile rinsing media, such as saline or Ringers solution, are available for such lavage procedures. In the decision of choosing the suitable rinsing solution, it is critical to balance the type of active ingredient, indications, physiological interactions, neutrality and cytotoxicity. In the current study, the effectiveness of physiological saline solutions and an experimental rinsing solution based on octenidine dihydrochloride was compared in relation to their germ reduction. Moreover, it is worthwhile to investigate to what extent an additional biological burden exists in the form of native blood and sand, how it is practical in transport accidents, the effects on the efficiency of the solutions and whether there exists a quantifiable difference between the test germs used with regard to their removal. Cross-sectioned sterilised pork hock pieces served as test surfaces; test germs from Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecium and Candida albicans were selected for contamination. On the whole, by using the octenidine-based solution, an average germ reduction of 2.1 log phase was achieved; the reduction when using saline solution was significantly lower at 1.4 log phase. The efficacy of both liquids could not limit an additional burden; in this case, the efficiency of octenidine dihydrochloride was clearly above that of isotonic saline solution. While octenidine could exhibit a similar microbiocidal efficacy to different germs, the saline solution had particular difficulty in removing the Gram-positive germ of Staphylococcus aureus. On account of their advantages as opposed to the frequently employed saline solution and octenidine tolerance described in the literature, the periodic application of octenidine-based wound-rinsing solutions should be taken into consideration.