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Entwicklung von diagnostischen Assays mit Magnetic Beads und frequenzmischungs-basierter Detektion
Basierend auf der Methode der Frequenzmischung zur Detektion von Magnetic Beads als Antikörper-konjugierte Labels mit kleinen Chromatographiehülsen wurden mehrere Assays entwickelt und verglichen. Hierbei wurden neben verschiedenen Probenmatrices wie z. B. Puffer, Serum und Lebensmitteln verschied...
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| Natura: | Dissertation |
| Lingua: | tedesco |
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Philipps-Universität Marburg
2008
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| Soggetti: | |
| Accesso online: | PDF Full Text |
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Based on the method of frequency-mixing for the detection of magnetic beads as antibody-conjugated labels with small chromatography columns, several assays were developed and compared. Several sample matrices as buffer, serum and food matrices were tested with sequential detection methods as well as with magnetic separation and a fluidic system. Additionally, several commercially available magnetic beads were tested for their usability in this detection system and compared.