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In vitro VEGF-Sekretion von malignen Schilddrüsenzelllinien:Einfluss von Wachstumsfaktoren und Untersuchung der Postrezeptorsignaltransduktion

Nach Stimulation von drei Schilddrüsenkarzinomzelllinien mit verschiedenen Wachstumsfaktoren wurde die VEGF-Sekretion mittels ELISA ermittelt. TSH war hier nicht der stärkste Stimulator. TGF-beta und EGF waren potenter. In Abwesenheit eines TSHr zeigten weitere Wachstumsfaktoren einen stimulativen E...

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Bibliographic Details
Main Author: Geyer, Vera Carina
Contributors: Zielke, Andreas (Prof. Dr.) (Thesis advisor)
Format: Doctoral Thesis
Language:German
Published: Philipps-Universität Marburg 2008
Subjects:
EGF
PKC
Medizin
Proteinkinase C
Thyreotropin
Thyrotropin
Epidermaler Wachstumsfaktor
Transforming Growth Factor beta
TGF beta
Medical sciences Medicine
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After stimulation of three thyroid cancer cell lines with several growth factors VEGF-secretion was analyzed by enzyme immunoassay.TSH was not the most powerfull stimulator of VEGF secretion. TGF-beta and EGF were more potent. In absence of a functional TSHr additional growth factors increase capacity for VEGF sectretion. TSHr signaling was evaluated by analyzing the effect of stimulators and inhibitors on VEGF protein levels under basal and TSH stimulated conditions. The effects of TSH were mediated by PKC rather than PKA.

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