Ermittlung der m-RNA-Expressionsmuster der c-jun, c-fos und c-myc Proto-Onkogene nach ex vivo Ballondilatation von Schweinekarotiden mittels semiquantitativer RT-PCR.

The goal of the present research study is to investigate the m-RNA-patterns of the proto oncogenes c-jun, c-myc and c-fos after balloon dilatation by semiquantitative RT-PCR in a porcine model. We generated successfully a simple and applicable method for this study. This method allowed us to determine short sequences of porcine c-myc and c-jun gene. We could not detect any change in m-RNA patterns due to a too small number of vessels used in this study. Nevertheless we saw a reduction in m-RNA expression of c-jun and an increase in m-RNA expression of c-fos after balloon dilatation. We could not find the so far describes increase in c-jun and c-myc gene expression. Additionally we could proof by a histological preparation of the vessels that dilatated and control-vessels are not damaged by dilatation and preparation. Further all vessel walls were found to be intact. There was no proof of denudation.