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Pharmakologische Charakterisierung der Wirkungen von Glukokortikoiden auf die Atmungskette und die ATP-Synthese in adrenokortikotrophen Hypophysenzellen (AtT20)
Um die Effekte des Glukokortikoids Dexamethason auf Parameter des mitochondrialen Energiestoffwechsels zu untersuchen, wurden in vitro Versuche an einer AtT20-Zelllinie durchgeführt (Hypophysenvorderlappenzellen der Maus). Die untersuchten Parameter waren die ATP-Konzentration und ein mitochondriale...
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| Format: | Doctoral Thesis |
| Language: | German |
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Philipps-Universität Marburg
2005
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| Online Access: | PDF Full Text |
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In order to characterize effects of the glucocorticoid dexamethasone on parameters of mitochondrial energy metabolism, we conducted in vitro trials, using a murine AtT20 cell line. The experimental parameters were the ATP-concentration and a mitochondrial function test (EZ4U). After longterm incubation (>30 min.), we found suppressing effects on the experimental parameters. After shortterm incubation (≤30 min.), the effects were either increasing or suppressing the experimental parameters, and dose-dependent. The shortterm effects in the mitochondrial function test could not be blocked by the glucocorticoid-receptor-antagonist RU38486. The effects observed are presumably nongenomic in nature and could be involved in regulation or impairment of the hypothalamic-pituitary-adrenocortical axis.