Comparative analysis of full-length 16s ribosomal RNA genome sequencing in human fecal samples using primer sets with different degrees of degeneracy

Comparative analysis of full-length 16s ribosomal RNA genome sequencing in human fecal samples using primer sets with different degrees of degeneracy

Next-generation sequencing has revolutionized the field of microbiology research and greatly expanded our knowledge of complex bacterial communities. Nanopore sequencing provides distinct advantages, combining costeffectiveness, ease of use, high throughput, and high taxonomic resolution through...

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Auteurs principaux: Wächter, Christian, Fehse, Leon, Welzel, Marius, Heider, Dominik, Babalija, Lek, Cheko, Juan, Müller, Julian, Pöling, Jochen, Braun, Thomas, Pankuweit, Sabine, Weihe, Eberhard, Kinscherf, Ralf, Schieffer, Bernhard, Luesebrink, Ulrich, Soufi, Muhidien, Ruppert, Volker
Format: Article
Langue:anglais
Publié: Philipps-Universität Marburg 2023
Sujets:
Medizin
gut microbiome
human fecal microbiome
oxford nanopore technologies (ONT)
primer degeneracy
next-generation sequencing (NGS), nanopore sequencing
MinION Mk1C
16S rRNA
Medical sciences Medicine
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