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Y-Complex Proteins Show RNA-Dependent Binding Events at the Cell Membrane and Distinct Single-Molecule Dynamics
Bacteria are dependent on rapid alterations in gene expression. A prerequisite for rapid adaptations is efficient RNA turnover, with endonuclease RNase Y playing a crucial role in mRNA stability as well as in maturation. In Bacillus subtilis, RNase Y in turn interacts with the so-called “Y-comple...
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| Päätekijät: | , , , |
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| Aineistotyyppi: | Artikkeli |
| Kieli: | englanti |
| Julkaistu: |
Philipps-Universität Marburg
2022
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| Aiheet: | |
| Linkit: | PDF-kokoteksti |
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| Yhteenveto: | Bacteria are dependent on rapid alterations in gene expression. A prerequisite for rapid
adaptations is efficient RNA turnover, with endonuclease RNase Y playing a crucial role in mRNA
stability as well as in maturation. In Bacillus subtilis, RNase Y in turn interacts with the so-called
“Y-complex” consisting of three proteins, which play important functions in sporulation, natural
transformation and biofilm formation. It is thought that the Y-complex acts as an accessory factor in
RNase Y regulation but might also have independent functions. Using single-molecule tracking, we
show that all three Y-complex proteins exhibit three distinct mobilities, including movement through
the cytosol and confined motion, predominantly at membrane-proximal sites but also within the cell
center. A transcriptional arrest leads to a strong change in localization and dynamics of YmcA, YlbF
and YaaT, supporting their involvement in global RNA degradation. However, Y-complex proteins
show distinguishable protein dynamics, and the deletion of yaaT or ylbF shows a minor effect on the
dynamics of YmcA. Cell fractionation reveals that YaaT displays a mixture of membrane association
and presence in the cytosol, while YlbF and YmcA do not show direct membrane attachment. Taken
together, our experiments reveal membrane-associated and membrane-independent activities of
Y-complex proteins and a dynamic interplay between them with indirect membrane association of
YmcA and YlbF via YaaT. |
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| Huomautukset: | Gefördert durch den Open-Access-Publikationsfonds der UB Marburg. |
| Ulkoasu: | 22 Seiten |
| DOI: | 10.3390/cells11060933 |