Structural and Functional Insight into the Mechanism of Bacillus subtilis 6S-1 RNA Release from RNA Polymerase

Structural and Functional Insight into the Mechanism of Bacillus subtilis 6S-1 RNA Release from RNA Polymerase

Here we investigated the refolding of Bacillus subtilis 6S-1 RNA and its release from σ A-RNA polymerase (σ A-RNAP) in vitro using truncated and mutated 6S-1 RNA variants. Truncated 6S-1 RNAs, only consisting of the central bubble (CB) flanked by two short helical arms, can still traverse the m...

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Principais autores: Ganapathy, Sweetha, Hoch, Philipp G., Lechner, Marcus, Bussiek, Malte, Hartmann, Roland K.
Formato: Artigo
Idioma:inglês
Publicado em: Philipps-Universität Marburg 2022
Assuntos:
Bacillus subtilis 6S-1 RNA
truncated and mutated derivatives
Medizin
6S-1 RNA:σA-RNAP release kinetics
product RNA (pRNA) transcription
gel shift assays;
Medical sciences Medicine
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