Structural and Functional Insight into the Mechanism of Bacillus subtilis 6S-1 RNA Release from RNA Polymerase

Structural and Functional Insight into the Mechanism of Bacillus subtilis 6S-1 RNA Release from RNA Polymerase

Here we investigated the refolding of Bacillus subtilis 6S-1 RNA and its release from σ A-RNA polymerase (σ A-RNAP) in vitro using truncated and mutated 6S-1 RNA variants. Truncated 6S-1 RNAs, only consisting of the central bubble (CB) flanked by two short helical arms, can still traverse the m...

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Autoren: Ganapathy, Sweetha, Hoch, Philipp G., Lechner, Marcus, Bussiek, Malte, Hartmann, Roland K.
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语言:英语
出版: Philipps-Universität Marburg 2022
主题:
Bacillus subtilis 6S-1 RNA
truncated and mutated derivatives
Medizin
6S-1 RNA:σA-RNAP release kinetics
product RNA (pRNA) transcription
gel shift assays;
Medical sciences Medicine
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索引号: urn:nbn:de:hebis:04-es2022-01561
Publikationsdatum: 2022-09-01
Quelle: Erstveröffentlichung: Ganapathy, S.; Hoch, P.G.; Lechner, M.; Bussiek, M.; Hartmann, R.K. Structural and Functional Insight into the Mechanism of Bacillus subtilis 6S-1 RNA Release from RNA Polymerase. Non-coding RNA 2022, 8, 20. https://doi.org/ 10.3390/ncrna8010020
Downloads: 27 (2024), 50 (2023), 3 (2022)
Lizenz: https://creativecommons.org/licenses/by/4.0
访问URL: https://archiv.ub.uni-marburg.de/es/2022/0156
https://doi.org/10.3390/ncrna8010020

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