Titel: | The Vibrio vulnificus stressosome is an oxygen-sensor involved in regulating iron metabolism |
Autor: | Heinz, Veronika |
Weitere Verfasser: | Jäckel, Wenke; Kaltwasser, Susann; Cutugno, Laura; Bedrunka, Patricia; Graf, Anica; Reder, Alexander; Michalik, Stephan; Dhople, Vishnu M.; Madej, M. Gregor; Conway, Maria; Lechner, Marcus; Riedel, Katharina; Banke, Gert; Boyd, Aoife; Völker, Uwe; Lewis, Richard J.; Marles-Wright, Jon; Ziegler, Christine; Pané-Farré, Jan |
Veröffentlicht: | 2022 |
URI: | https://archiv.ub.uni-marburg.de/es/2023/0131 |
DOI: | https://doi.org/10.1038/s42003-022-03548-w |
URN: | urn:nbn:de:hebis:04-es2023-01310 |
DDC: | 540 Chemie |
Publikationsdatum: | 2023-09-04 |
Lizenz: | https://creativecommons.org/licenses/by/4.0 |
Schlagwörter: |
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Summary:
Stressosomes are stress-sensing protein complexes widely conserved among bacteria. Although a role in the regulation of the general stress response is well documented in Gram-positive bacteria, the activating signals are still unclear, and little is known about the physiological function of stressosomes in the Gram-negative bacteria. Here we investigated the stressosome of the Gram-negative marine pathogen Vibrio vulnificus. We demonstrate that it senses oxygen and identified its role in modulating iron-metabolism. We determined a cryo-electron microscopy structure of the VvRsbR:VvRsbS stressosome complex, the first solved from a Gram-negative bacterium. The structure points to a variation in the VvRsbR and VvRsbS stoichiometry and a symmetry breach in the oxygen sensing domain of VvRsbR, suggesting how signal-sensing elicits a stress response. The findings provide a link between ligand-dependent signaling and an output – regulation of iron metabolism - for a stressosome complex.
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