Titel: | Protective CD8+ T Cell Response Induced by Modified Vaccinia Virus Ankara Delivering Ebola Virus Nucleoprotein |
Autor: | Kupke, Alexandra |
Weitere Verfasser: | Dietzel, Erik; Schmidt, Jörg; Shams-Eldin, Hosam; Sauerhering, Lucie; Krähling, Verena; Gellhorn Serra, Michelle; Eickmann, Markus; Becker, Stephan |
Veröffentlicht: | 2023 |
URI: | https://archiv.ub.uni-marburg.de/es/2023/0005 |
DOI: | https://doi.org/10.3390/vaccines10040533 |
URN: | urn:nbn:de:hebis:04-es2023-00054 |
DDC: | 610 Medizin |
Publikationsdatum: | 2023-03-06 |
Lizenz: | https://creativecommons.org/licenses/by/4.0 |
Schlagwörter: |
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correlates of protection; nucleoprotein, vaccine, Modified Vaccinia virus Ankara, Ebola virus, glycoprotein |
Summary:
The urgent need for vaccines against Ebola virus (EBOV) was underscored by the large
outbreak in West Africa (2014–2016). Since then, several promising vaccine candidates have been
tested in pre-clinical and clinical studies. As a result, two vaccines were approved for human use
in 2019/2020, of which one includes a heterologous adenovirus/Modified Vaccinia virus Ankara
(MVA) prime-boost regimen. Here, we tested new vaccine candidates based on the recombinant
MVA vector, encoding the EBOV nucleoprotein (MVA-EBOV-NP) or glycoprotein (MVA-EBOV-GP)
for their efficacy after homologous prime-boost immunization in mice. Our aim was to investigate
the role of each antigen in terms of efficacy and correlates of protection. Sera of mice vaccinated
with MVA-EBOV-GP were virus-neutralizing and MVA-EBOV-NP immunization readily elicited
interferon-
-producing NP-specific CD8+ T cells. While mock-vaccinated mice succumbed to EBOV
infection, all vaccinated mice survived and showed drastically decreased viral loads in sera and
organs. In addition, MVA-EBOV-NP vaccinated mice became susceptible to lethal EBOV infection
after depletion of CD8+ T cells prior to challenge. This study highlights the potential of MVA-based
vaccines to elicit humoral immune responses as well as a strong and protective CD8+ T cell response
and contributes to understanding the possible underlying mechanisms.
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