Dokument

Summary:
Abstract There are a lot of examples and contradictory results concerning influence of low intensity laser irradiation (LILI) on biological objects. In this work with a number of experiments the influence of LILI on different biological systems was investigated. For the carried out experiments the following biological objects and systems were used: * different enzymes of anti-oxidant system of animals (i.e. catalase, superoxide-dismutase, glutathion-peroxidase, glutathion-reductase) * Mg2+- and Ca2+-ATPase of membrane from human erythrocytes; as well as from rats' ones * lactat- and succinat-dehydrogenase from rat's tissues liver, kidney, brain, muscle, heart * alpha-amylase from dry and germinated seeds of barley * manganese-peroxidase from lignin-destroying mushrooms * dehydrogenase system of yeast * dehydrogenase system of bacteria The following laser equipment was set up: YAG-Laser (355 nm and 533 nm), Argon-Laser (458 nm, 488 nm and 520 nm), Helium-Neon-Laser (632 nm) and CO2-Laser (10.6 µm). Activity of different enzymatic systems was detected after laser-light treatment to compare with reading of their activity without radiation. Depending on laser light intensity, time of exposition and own properties of system different biological responses were detected. Besides that the kind of laser (its wave length) causes a variety of reactions in a system.

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