Elektrophysiologische Charakterisierung des klassischen TRP-Kanals TRPC5

Klassische TRP-Kanäle (TRPC) sind Ca(2+)-permeable, unselektive Kationenkanäle, die durch PLC-abhängige Signalwege intrazellulär aktiviert werden. Obwohl die ersten Mitglieder dieser Subfamilie bereits vor über 15 Jahren kloniert wurden, sind sie auf funktioneller Ebene bisher nur unvollständig unte...

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Bibliographic Details
Main Author: Semtner, Marcus
Contributors: Plant, Timothy (Prof. Dr.) (Thesis advisor)
Format: Doctoral Thesis
Published: Philipps-Universität Marburg 2010
Online Access:PDF Full Text
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Classical TRP channels (TRPC) are Ca(2+)-permeable, unselective cation channels that are activated by PLC-dependent intracellular pathways. Although most members of this subfamily were cloned at least 10 years ago, functional data on some of their biophysical properties are still not available. The main aim of this study was to investigate the properties of TRPC5, and to compare them with those of other members of the TRPC subfamily. In the present study, TRPC5 was characterised to be a voltage-dependent channel that can be modulated directly by a multiplicity of extracellular elementary cations. The permeation of mono- or divalent cations through TRPC5 is unselective and determined by amino acids between the 5th and 6th transmembrane domain.