Generierung und Charakterisierung eines neuen Donorstammes für die Influenza Saatvirusherstellung und die Charakterisierung von Influenza Viren in verschiedenen Wirtssystemen

In der Influenza-Impfstoffproduktion ist es von essentieller Bedeutung, gewünschte Antigene (Hämagglutinin und Neuraminidase) in möglichst kurzer Zeit zu erhalten, damit die Bevölkerung gegen die kommenden Influenzaviren schnell und bestmöglich geschützt ist. Derzeit werden die Saatviren in Eiern he...

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Bibliographic Details
Main Author: Bertram, Sybille
Contributors: Bölker, Michael (Prof.) (Thesis advisor)
Format: Doctoral Thesis
Published: Philipps-Universität Marburg 2010
Online Access:PDF Full Text
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Table of Contents: In Vaccine production it is essential to obtain the wanted antigens (HA and NA) in a short time, to protect the population from circulating influenzaviruses. When human influenzaviruses are incubated on eggs they obtain mutations in HA and NA which can lead to unsatisfactory vaccination efficacies. To avoid such mutations it is an atvantage to use cell culture. Furthermore it is important to produce the highest amount of virus in shorter times. For this purpose a new donorvirus was established which showd the best groth on the MDCK33016PF suspension cell line. Comparison with the commonly used PR8 Donorstrain revealed that the new established NB # 105 Donorstrain ist superior in growth on MDCK33016PF cells and on Eggs. Mutational analysis showed that the growth is applicable when NA and HA are exchanged and better than PR8.