The ParA-like protein AgmE positively regulates cell division in Myxococcus xanthus
Correct positioning of the division plane is a prerequisite for the generation of daughter cells with a normal chromosome complement and with a correct size. So far, all bacterial systems, which contribute to correctly place the division plane, regulate formation of the FtsZ cytokinetic ring at m...
|PDF Full Text
No Tags, Be the first to tag this record!
|Correct positioning of the division plane is a prerequisite for the generation
of daughter cells with a normal chromosome complement and with a correct
size. So far, all bacterial systems, which contribute to correctly place the
division plane, regulate formation of the FtsZ cytokinetic ring at mid-cell.
Myxococcus xanthus belongs to the δ-proteobacteria and divides by binary
fission. However, the mechanisms that ensure proper cell division are not
known. In contrast, M. xanthus has been studied in detail because of its
complex life cycle and for its gliding motility. M. xanthus possesses two
motility systems referred to as the social (S) and the adventurous (A)-
motility systems. While analyzing the adventurous gliding motility E protein
(AgmE), which is a member of the ParA/Soj family of ATPases, we
discovered that an in-frame deletion of the agmE gene results in the
formation of filamentous cells and chromosome-free mini-cells. We
determined that an ΔagmE mutant is neither impaired in chromosome
replication nor in chromosome segregation. Moreover, an ΔagmE mutant
cells displayed fewer division sites and frequently these sites were not
located to mid-cell. These data strongly suggest that AgmE has a central
function in cell division and in placing the division plane at mid-cell.
Consequently, FtsZ localization was investigated. In WT, FtsZ localizes in a
speckled-pattern in small-sized cells, i.e. early in the cell cycle, whereas in
larger cells, i.e. later in the cell cycle, FtsZ localizes at mid-cell. On the
contrary, in an ΔagmE mutant FtsZ localizes in a speckled-pattern
independently of cell length and cell cycle. We hypothesized that AgmE is
required to properly localize FtsZ and could act either positively by directing
FtsZ to mid-cell or by stabilizing the FtsZ cytokinetic ring or negatively by
inhibiting FtsZ cytokinetic ring formation at the poles.To distinguish between these hypotheses, AgmE localization was studied.
AgmE was found to localize in three distinct patterns, which correlates with
cell length, i.e. the cell cycle. In small-sized cells, AgmE localizes in a
patchy pattern, as cell size increases AgmE localizes to a single focus
slightly off mid-cell and when the cells have reached a specific cell length AgmE localizes at mid-cell. Genetic data suggest that the cell cycle
dependent localization of AgmE depends on ATPase activity. The localization
of AgmE at mid-cell implies that AgmE acts positively on FtsZ localization or
stabilizes the FtsZ cytokinetic ring. To distinguish between these models,
co-localization studies were carried out. These analyses demonstrated that
FtsZ and AgmE co-localize at mid-cell. Intriguingly, some cells displayed
mid-cell localization of AgmE in the absence of mid-cell localization of FtsZ.
Moreover, in vitro analyses showed that AgmE interact directly with FtsZ.
On the basis of the localization of AgmE to mid-cell and the observation that
AgmE localize to mid-cell before FtsZ, we propose that AgmE is a novel cell
division regulator that acts positively to direct FtsZ to mid-cell.
A systematic analysis of the M. xanthus proteome revealed a unique
combination of cell division regulators, i.e. M. xanthus encodes an
orthologue of DivIVA and lacks orthologues of MinCDE, MipZ, SlmA and Noc.
These observations suggest that cell division regulators are yet to be
discovered in M. xanthus. In this study, we have shown that M. xanthus
possesses the orphan ParA-like protein AgmE, which is involved in cell
division. Importantly, AgmE is the first example of a protein shown to
positively regulate FtsZ localization. Interestingly, an in-frame deletion of
divIVA has no obvious effect on cell division and a DivIVA-mCherry protein
localizes in a speckled pattern. Moreover, MXAN0636, the downstream gene
of agmE, seems to be an additional component for the regulation of cell
division in M. xanthus.