Expression und Regulation von Typ II transmembranären Serin Proteasen (TTSP) auf humanen intestinalen Epithelzellen

Die Entdeckung und Untersuchung der Typ II transmembranären Serin Proteasen (TTSP) befindet sich noch immer in den Anfängen und obwohl erste Schritte schon gemacht wurden, bleibt noch ein weiter Weg, bis alle Fragen beantwortet sind. Dabei verschafft ihnen gerade die Lokalisation an der Zelloberfläc...

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Bibliographic Details
Main Author: Siebert, Marcus Rudi
Contributors: Böhm, Stephan (Dr.) (Thesis advisor)
Format: Doctoral Thesis
Published: Philipps-Universität Marburg 2009
Online Access:PDF Full Text
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Table of Contents: Type II transmembrane serine proteases (TTSP) are a new and expanding family of proteolytic enzymes. Currently little is known about their physiological functions, although it was shown that many TTSP are overexpressed in human tumors. In this study the expression and regulation of four TTSP (namely MT-SP1, Hepsin, TMPRSS-2, TMPRSS-4) on human intestinal epithelial cells using a real-time PCR was rewied. It could be shown that TTSP and PAR-2 are coexpressed in human gastrointestinal tissues and this with highest levels around coecum. In further inflammatory cell model studies using cytokines and different bacterial strains TTSP expression was detected. Interestingly Entercoccus faecium induced a considerable downregulation of expression. In contrast E. coli Nissle as a probiotic strain induced a strong overexpression. Different preparations of E. coli exhibit that the heat-inactivated preparation induced highest overexpression.