Konstruktion und Charakterisierung einer Mutante des GPI-Biosyntheseweges von Plasmodium falciparum

Gylkosylphosphatidylinositole sind eine Klasse von Glykolipiden, die bei allen Eukaryonten vorkommen. Die Biosynthese der GPI-Anker erfolgt durch schrittweise Übertragung der einzelnen Zuckerkomponenten auf Phosphatidylinositol. Anschließend wird der GPI-Anker auf das C-terminale Ende eines neu synt...

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Bibliographic Details
Main Author: Dorn, Caroline Julia Marlies
Contributors: Schwarz, R.T. (Prof.) (Thesis advisor)
Format: Doctoral Thesis
Published: Philipps-Universität Marburg 2009
Online Access:PDF Full Text
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Table of Contents: Glycosylphosphatidylinositol represents a complex glycolipid which plays an important role as anchoring molecule for cell surface proteins. The biosynthesis of these molecules is a complex process which involves step-by-step transfer of single sugar components to phosphytidylinositol. This work concentrates on dolichol phosphate mannose synthase of Plasmodium falciparum an enzyme which shows its activity in the ER compartment. The known sequences of dolichol phosphate mannose synthases (DPM1) are divided into two groups. To achieve further information about the DPM1-protein of P. falciparum, mutants of this organism were made which show different marker sequences at the DPM1 protein. The sequence analysis of this protein, the fact that a hydrophobic marker sequence seems to interfere with the hydrophilic DPM1-protein and the failing of finding homologous subunits to its human counterpart lead to the conclusion that the parasites protein is unique among the DPM-synthases and could open a new field of antimalarial therapy.