Konstruktion einer rekombinanten Phage-Display-Antikörper Bibliothek zur Isolierung von humanen monoklonalen Antikörpern gegen Lassavirus und Plasmodium falciparum
Ziel der Arbeit war die Etablierung der Phage-Display-Technik im Labor der Arbeitsgruppe von Herrn Dr.med. J. ter Meulen. Es wurde eine Antikörper-Phagen-Bibliothek aus den Lymphozeten eines Lassa und Plasmodium falciparum seropositiven Spender generiert. Aus dieser sollten gegen Modelantigene (Lass...
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| Format: | Doctoral Thesis |
| Language: | German |
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Philipps-Universität Marburg
2008
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| Online Access: | PDF Full Text |
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The aim of this thesis was the establishment of the phage-display technique in the lab of Dr. J. ter Meulen. An antibody-phage-display library was generated from the lymphocytes of a lassa and plasmodium falciparum seropositiv donor. From this monoclonal antibodies in the form of scFv displaying phages should be isolated targeting model antigens (lassa virus, lassa virus GP2, plasmodium falciparum infected erythrocytes and plasmodium falciparum Pff2). The generated library consisted of 5,25 million clones and was thus quite small. Enrichment of antibodies against infected erythrocytes only could beshown, however the original library showed bindung to lassa virus and Pff2 in ELISA and to infected erythrocytes in flow cytometry.