Vergleichende Untersuchung zum Nachweis von ausgewählten parodontalpathogenen Mikroorganismen mittels konventioneller Kultur versus DNA-CHip bei Patienten mit Arteriosklerose-Verdacht

Summary In this study 112 patients, 83 with a coronary heart disease (CHD +) and 29 without a coronary heart disease (CHD -), were investigated. Sulcus fluid was taken from all patients with sterile paper points. These subgingival microbial samples were analyzed in the Institute of Medical Microbiology and Hygiene to prove the occurrence of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia and Peptostreptoccus micros. These four microorganisms are responsible for parodontal diseases. The microorganisms were proven with two techniques, microbiological culture and a new DNA-Chip. The microorganisms were hybridised at microscopic small points, the zone of hybridisation, on the slide, named chip. As a result, a higher number of microorganisms could be shown with the chip. A significant correlation between coronary heart disease and the presence of the micoorganisms could not be shown. Between the occurrence of the microorganisms and the age or the sex of the patients no correlation has been found. The DNA chip exhibits the possibility to analyze 20 different microorganisms at once; it was here just a marginal release, but the most probes have shown 11 to 15 microorganisms. The results of this study show the necessity of further examinations to consider the advantages of the chip. The chip seems to be safer in pointing out the microorganisms than the cultures because of less sources of error. It is also possible to find a reliable conclusion to the real contract of the disease with a semiquantitave determination. The results show, that more examinations are necessary to validate the chips quality. It is also important to proof the chips effieciency. The chip could be an alternative method, at least it could be a support or supplement for the clinical diagnostics.