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Elektrogesponnene Polymerfasern:Funktionalisierung und Einsatz im Bone Tissue Engineering
Die vorliegende Arbeit beschäftigt sich mit der Herstellung und Modifikation von elektrogesponnenen Mikro- und Nanofasern sowie Geräten zu ihrer Herstellung für verschiedene Anwendungsbereiche, wie den Einsatz im Bone Tissue Engineering oder in der Aufbringung auf Pflanzen. Hierzu wurden zunächst...
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| Format: | Doctoral Thesis |
| Language: | German |
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Philipps-Universität Marburg
2006
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| Online Access: | PDF Full Text |
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In this work the production and modification of electrospun micro- and nanofibers and the construction of electrospinning setups for various applications like bone tissue engineering or deposition on plants is described. The conditions for the production of a fiber matrix were developed and in a model system with human osteosarcome cells the suitability of this matrix structure for tissue engineering was investigated. In a next step the growth and differentiation behavior of human mesenchymal stem cells on a matrix of poly-l-lactide fibers was investigated and a osteogenic differentiation was observed. The electrospinning setup was modified to obtain a structured fiber matrix that was tested as a model for a three-dimensional matrix in a flow-through system with osteosarcome cells. Several biocompatible and biodegradable polymers were spun and tested as matrix material for human mesenchymal stem cells. With coaxial electrospinning core-shell fibers of different polymers and the incorporation of green fluorescent protein could be obtained.