ATP induzierte Membranpotenzial-Oszillationen in humanen Makrophagen

Die Doktorarbeit zeigt, dass ATP in mikromolaren Konzentrationen bei humanen Makrophagen Oszillationen des Membranpotentials und des intrazellulären Kalziums auslöst. Diese Oszillationen wirken auf die Zytokinproduktion der Makrophagen und erhöhen die Produktion von IL-6. Des weitern konnten in dies...

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Bibliographic Details
Main Author: Musset, Boris
Contributors: Stengl, Monika (Prof.) (Thesis advisor)
Format: Doctoral Thesis
Published: Philipps-Universität Marburg 2005
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The effects of low concentrations of extracellular ATP on cytosolic Ca2+, membrane potential, and transcription of IL-6 were studied in monocyte-derived human macrophages. During inflammation or infection many cells secrete ATP. Application of 10 µM ATP induces oscillations in cytosolic Ca2+ with a frequenzy of 12 min-1 and oscillations in membrane potential. Purinreceptors of macrophages were classified and also potassiumchannels could be identified which take part in the oscillations. The production of IL-6 is 20 times higher during the application of ATP. This findings suggest that ATP is a danger signal for innate immune system.