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Polyethylenimine and its derivates: investigation of in vivo fate, subcellular trafficking and development of novel vector systems
In this dissertation several aspects of polymer based gene delivery were investigated. First, key issues in subcellular processing of electrostatic polymer/nucleic acid complexes were investigated and new insights into mechanisms involved in these processes were gained. Secondly, a...
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| Main Author: | |
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| Format: | Doctoral Thesis |
| Language: | English |
| Published: |
Philipps-Universität Marburg
2003
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| Subjects: | |
| Online Access: | PDF Full Text |
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| Summary: | In this dissertation several aspects of polymer
based gene delivery were investigated. First, key issues in
subcellular processing of electrostatic polymer/nucleic acid
complexes were investigated and new insights into mechanisms
involved in these processes were gained.
Secondly, a targeted
gene delivery system was developed for the specific
transfection of ovarian carcinoma cells. The resulting vector
exhibited a high specificity for target cells combined with low
unspecific transfection and toxicity. Furthermore, a novel type
of gene delivery system was synthesized. This vector exhibited
a high in vitro transfection efficiency and a very low in vitro
toxicity as well as favourable in vivo properties, such as
reduced toxicities. Another aspect that was studied in depth
was the investigation of the stability of several electrostatic
vectors in vitro and when applied intravenously. |
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| Physical Description: | 241 Pages |
| DOI: | 10.17192/z2003.0651 |