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Therapeutic Treatment of Superoxide Dismutase 1 (G93A) Amyotrophic Lateral Sclerosis Model Mice with Medical Ozone Decelerates Trigeminal Motor Neuron Degeneration, Attenuates Microglial Proliferation, and Preserves Monocyte Levels in Mesenteric Lymph Nodes
Amyotrophic lateral sclerosis (ALS) is an incurable and lethal neurodegenerative disease in which progressive motor neuron loss and associated inflammation represent major pathology hallmarks. Both the prevention of neuronal loss and neuro-destructive inflammation are still unmet challenges. Medi...
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| Autoren: | , , , |
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| Format: | Artikel |
| Sprache: | Englisch |
| Veröffentlicht: |
Philipps-Universität Marburg
2022
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| Online Zugang: | PDF-Volltext |
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| Zusammenfassung: | Amyotrophic lateral sclerosis (ALS) is an incurable and lethal neurodegenerative disease
in which progressive motor neuron loss and associated inflammation represent major pathology
hallmarks. Both the prevention of neuronal loss and neuro-destructive inflammation are still unmet
challenges. Medical ozone, an ozonized oxygen mixture (O3/O2), has been shown to elicit profound
immunomodulatory effects in peripheral organs, and beneficial effects in the aging brain. We investigated,
in a preclinical drug testing approach, the therapeutic potential of a five-day O3/O2 i.p.
treatment regime at the beginning of the symptomatic disease phase in the superoxide dismutase
(SOD1G93A) ALS mouse model. Clinical assessment of SOD1G93A mice revealed no benefit of medical
ozone treatment over sham with respect to gross body weight, motor performance, disease duration,
or survival. In the brainstem of end stage SOD1G93A mice, however, neurodegeneration was found
decelerated, and SOD1-related vacuolization was reduced in the motor trigeminal nucleus in the
O3/O2 treatment group when compared to sham-treated mice. In addition, microglia proliferation
was less pronounced in the brainstem, while the hypertrophy of astroglia remained largely unaffected.
Finally, monocyte numbers were reduced in the blood, spleen, and mesenteric lymph nodes at postnatal
day 60 in SOD1G93A mice. A further decrease in monocyte numbers seen in mesenteric lymph
nodes from sham-treated SOD1G93A mice at an advanced disease stage, however, was prevented
by medical ozone treatment. Collectively, our study revealed a select neuroprotective and possibly
anti-inflammatory capacity for medical ozone when applied as a therapeutic agent in SOD1G93A
ALS mice.
Keywords: amyotrophic |
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| Beschreibung: | Gefördert durch den Open-Access-Publikationsfonds der UB Marburg. |
| Umfang: | 16 Seiten |
| DOI: | 10.3390/ijms23063403 |