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Involvement of E. coli 6S RNA in Oxidative Stress Response
6S RNA, a small non-coding RNA present in almost all bacteria, inhibits transcription via direct binding to RNA polymerase holoenzymes. The mechanism of 6S RNA action was investigated to a large extent in E. coli, however, lack of 6S RNA (DssrS) was demonstrated to be unfavorable but not essentia...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Philipps-Universität Marburg
2022
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| Subjects: | |
| Online Access: | PDF Full Text |
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| Summary: | 6S RNA, a small non-coding RNA present in almost all bacteria, inhibits transcription via
direct binding to RNA polymerase holoenzymes. The mechanism of 6S RNA action was investigated
to a large extent in E. coli, however, lack of 6S RNA (DssrS) was demonstrated to be unfavorable but
not essential for cell survival under various growth conditions. In the present study, we revealed, for
the first time, a lethal phenotype of the DssrS strain in the presence of high concentrations of H2O2.
This phenotype was rescued by complementation of the ssrS gene on a plasmid. We performed
comparative qRT-PCR analyses on an enlarged set of mRNAs of genes associated with the oxidative
stress response, allowing us to identify four genes known to be involved in this pathway (soxS, ahpC,
sodA and tpx) that had decreased mRNA levels in the DssrS strain. Finally, we performed comparative
proteomic analyses of the wild-type and DssrS strains, confirming that DssrS bacteria have reduced
levels of the proteins AhpC and Tpx involved in H2O2 reduction. Our findings substantiate the
crucial role of the riboregulator 6S RNA for bacterial coping with extreme stresses. |
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| Item Description: | Gefördert durch den Open-Access-Publikationsfonds der UB Marburg. |
| Physical Description: | 16 Pages |
| DOI: | 10.3390/ijms23073653 |