Assessing the Dermal Penetration Efficacy of Chemical Compounds with the Ex-Vivo Porcine Ear Model
(1) Background: The ex vivo porcine ear model is often used for the determination of the dermal penetration efficacy of chemical compounds. This study investigated the influence of the post-slaughter storage time of porcine ears on the dermal penetration efficacy of chemical compounds. (2) Method...
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|(1) Background: The ex vivo porcine ear model is often used for the determination of the
dermal penetration efficacy of chemical compounds. This study investigated the influence of the
post-slaughter storage time of porcine ears on the dermal penetration efficacy of chemical compounds.
(2) Methods: Six different formulations (curcumin and different fluorescent dyes in different vehicles
and/or nanocarriers) were tested on ears that were (i) freshly obtained, (ii) stored for 24 or 48 h at 4 �C
after slaughter before use and (iii) freshly frozen and defrosted 12 h before use. (3) Results: Results
showed that porcine ears undergo post-mortem changes. The changes can be linked to rigor mortis
and all other well-described phenomena that occur with carcasses after slaughter. The post-mortem
changes modify the skin properties of the ears and affect the penetration efficacy. The onset of rigor
mortis causes a decrease in the water-holding capacity of the ears, which leads to reduced penetration
of chemical compounds. The water-holding capacity increases once the rigor is released and results
in an increased penetration efficacy for chemical compounds. Despite different absolute penetration
values, no differences in the ranking of penetration efficacies between the different formulations
were observed between the differently aged ears. (4) Conclusions: All different types of ears can be
regarded to be suitable for dermal penetration testing of chemical compounds. The transepidermal
water loss (TEWL) and/or skin hydration of the ears were not correlated with the ex vivo penetration
efficacy because both an impaired skin barrier and rigor mortis cause elevated skin hydration and
TEWL values but an opposite penetration efficacy. Other additional values (for example, pH and/or
autofluorescence of the skin) should, therefore, be used to select suitable and non-suitable skin
areas for ex vivo penetration testing. Finally, data from this study confirmed that smartFilms and
nanostructured lipid carriers (NLC) represent superior formulation strategies for efficient dermal and
transdermal delivery of curcumin.
|Gefördert durch den Open-Access-Publikationsfonds der UB Marburg.