Dokument

Zusammenfassung:
Der Misserfolg einer Wurzelkanalbehandlung hat viele Ursachen, die nicht immer eindeutig sind. Dem Behandler stehen zahlreiche Herangehensweisen und Medikamente zur Verfügung, um eine endodontische Behandlung durchzuführen. Sie umfasst das mechanische Aufbereiten des Kanals, das Spülen mit geeigneter Flüssigkeit sowie einen bakterien- und flüssigkeitsdichten Verschluss. Zum einen sollte dieses Material eine Haftkraft zum umgebenden Wurzeldentin ausbilden, zum anderen sollte zum gewählten Kernmaterial ebenfalls eine Adhäsion entstehen [101]. In dieser In-vitro-Studie sollte der Einfluss verschiedener Spüllösungen auf den Haftverbund zwischen Wurzelkanaldentin und dem calciumsalicylatbasierten Sealers Apexit Plus untersucht werden. In dieser Studie wurden 60 extrahierte, kariesfreie und unbehandelte Zahnwurzeln auf fünf Gruppen randomisiert verteilt (n=12). Sie wurden dekapitiert und mit dem maschinellen Feilensystem BioRaCe bei einer Arbeitslänge von 8 mm bis .02#60 aufbereitet. Die zuvor gebildeten Gruppen wurden einer Spüllösung zugeteilt: A = Chlorhexidindiglukonat (CHX) 2%, B = Ethylendiamintetraacetat (EDTA) 16%, C = Natriumhypochlorit (NaOCl) 3%, D = Zitronensäure 40%, E = Aqua dest. Nachdem die Proben mit ihrer zugeordneten Flüssigkeit gespült wurden, erfolgte die sorgfältige Trocknung mit Papierspitzen und anschließendem Einbringen des vorbereiteten Stahlspreaders mit dem zu untersuchenden Sealer. Die Wurzeln lagerten 14 Tage unter feuchten Bedingungen. Anschließend fanden die Pullout-Tests in einer Universalprüfmaschine statt. Es wurde die maximale Kraft bis zum adhäsiven Versagen bei einer Prüfgeschwindigkeit von 2 mm/min ermittelt. Zusätzlich erfolgte eine Auswertung der Frakturmodi an den experimentellen Spreadern. Apexit Plus zeigte in Kombination mit CHX (0,73 MPa) und NaOCl (0,62 MPa) die höchsten Haftwerte. Nach einer Spülung mit EDTA zeigten sich insgesamt die geringsten Haftwerte (0,21 MPa). Es ergab sich ein signifikanter Einfluss der Spülungen auf die Haftwerte (Kruskal-Wallis Test p= 0,034). Unter den Bedingungen dieser Studie profitierte der Sealer Apexit Plus nicht von der Schmierschichtentfernung durch EDTA. Die Spülung führte zu geringen Haftwerten. Der calciumsalicylatbasierte Sealer verhielt sich somit gegensätzlich zu den meisten anderen Wurzelkanalsealern, die in der Regel zu besseren Haftwerten nach Schmierschichtentfernung gelangen. Basierend auf den Ergebnissen dieser Studie mit Apexit Plus ist eine alleinige Spülung mit EDTA nicht empfehlenswert. Ob Apexit Plus eine Verbesserung der Haftkraft durch eine zusätzliche Spülung mit CHX erlangen kann, war nicht Ziel dieser Arbeit und ist anderweitig zu erforschen.

Bibliographie / References

1. Wu MK, Wesselink PR, Boersma J (1995) A 1-year follow-up study on leakage of four root canal sealers at different thicknesses. Int Endod J 28:185- 189
2. Badole GP, Warhadpande MM, Meshram GK et al. (2013) A comparative evaluation of cytotoxicity of root canal sealers: an in vitro study. Restor Dent Endod 38:204-209
3. Takeda FH, Harashima T, Kimura Y et al. (1999) A comparative study of the removal of smear layer by three endodontic irrigants and two types of laser. Int Endod J 32:32-39
4. Van Noort R, Noroozi S, Howard IC et al. (1989) A critique of bond strength measurements. J Dent 17:61-67
5. Teixeira CS, Alfredo E, Thomé LH et al. (2009) Adhesion of an endodontic sealer to dentin and gutta-percha: shear and push-out bond strength measurements and SEM analysis. J Appl Oral Sci 17:129-135
6. Fidel RA, Sousa Neto MD, Spanó JC et al. (1994) Adhesion of calcium hydroxide-containing root canal sealers. Braz Dent J 5:53-57
7. Saleh IM, Ruyter IE, Haapasalo MP et al. (2003) Adhesion of endodontic sealers: scanning electron microscopy and energy dispersive spectroscopy. J Endod 29:595-601
8. Gettleman BH, Messer HH, ElDeeb ME (1991) Adhesion of sealer cements to dentin with and without the smear layer. J Endod 17:15-20
9. Braga RR, Meira JB, Boaro LC et al. (2010) Adhesion to tooth structure: a critical review of "macro" test methods. Dent Mater 26:e38-49
10. Bajaj D, Sundaram N, Nazari A et al. (2006) Age, dehydration and fatigue crack growth in dentin. Biomaterials 27:2507-2517
11. Kinney JH, Nalla RK, Pople JA et al. (2005) Age-related transparent root dentin: mineral concentration, crystallite size, and mechanical properties. Biomaterials 26:3363-3376
12. Eldeniz AU, Ørstavik D (2009) A laboratory assessment of coronal bacterial leakage in root canals filled with new and conventional sealers. Int Endod J 42:303-312
13. Wu MK, Tigos E, Wesselink PR (2002) An 18-month longitudinal study on a new silicon-based sealer, RSA RoekoSeal: a leakage study in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 94:499-502
14. Webber MB, Michida SM, Marson FC et al. (2015) Analysis of bond strength by pull out test on fiber glass posts cemented in different lengths. J Int Oral Health 7:7-12
15. Shipper G, Ørstavik D, Teixeira FB et al. (2004) An evaluation of microbial leakage in roots filled with a thermoplastic synthetic polymer-based root canal filling material (Resilon). J Endod 30:342-347
16. Jacobsen EL, BeGole EA, Vitkus DD et al. (1987) An evaluation of two newly formulated calcium hydroxide cements: a leakage study. J Endod 13:164-169
17. Kahn FH, Rosenberg PA, Schertzer L et al. (1997) An in-vitro evaluation of sealer placement methods. Int Endod J 30:181-186
18. Prestegaard H, Portenier I, Ørstavik D et al. (2014) Antibacterial activity of various root canal sealers and root-end filling materials in dentin blocks infected ex vivo with Enterococcus faecalis. Acta Odontol Scand 72:970-976
19. Tanomaru JM, Tanomaru-Filho M, Hotta J et al. (2008) Antimicrobial activity of endodontic sealers based on calcium hydroxide and MTA. Acta Odontol Latinoam 21:147-151
20. Mohammadi Z, Abbott PV (2009) Antimicrobial substantivity of root canal irrigants and medicaments: a review. Aust Endod J 35:131-139
21. Miletić I, Ribarić SP, Karlović Z et al. (2002) Apical leakage of five root canal sealers after one year of storage. J Endod 28:431-432
22. Herbert J, Bruder M, Braunsteiner J et al. (2009) Apical quality and adaptation of Resilon, EndoREZ, and Guttaflow root canal fillings in combination with a noncompaction technique. J Endod 35:261-264
23. McComb D, Smith DC (1975) A preliminary scanning electron microscopic study of root canals after endodontic procedures. J Endod 1:238-242
24. Cobankara FK, Adanir N, Belli S et al. (2002) A quantitative evaluation of apical leakage of four root-canal sealers. Int Endod J 35:979-984
25. Gluskin AH, Brown DC, Buchanan LS (2001) A reconstructed computerized tomographic comparison of Ni-Ti rotary GT files versus traditional instruments in canals shaped by novice operators. Int Endod J 34:476-484
26. Khullar P, Raisingani D, Gupta S et al. (2013) A survey report on effect of root canal fillings and coronal restorations on the periapical status of endodontically treated teeth in a selected group of population. Int J Clin Pediatr Dent 6:89-94
27. Timpawat S, Amornchat C, Trisuwan WR (2001) Bacterial coronal leakage after obturation with three root canal sealers. J Endod 27:36-39
28. Dalton BC, Orstavik D, Phillips C et al. (1998) Bacterial reduction with nickel- titanium rotary instrumentation. J Endod 24:763-767
29. Drake DR, Wiemann AH, Rivera EM et al. (1994) Bacterial retention in canal walls in vitro: effect of smear layer. J Endod 20:78-82
30. Kaur A, Shah N, Logani A et al. (2015) Biotoxicity of commonly used root canal sealers: A meta-analysis. J Conserv Dent 18:83-88
31. Ebert J, Leyer A, Günther O et al. (2011) Bond strength of adhesive cements to root canal dentin tested with a novel pull-out approach. J Endod 37:1558-1561
32. Hermann, BW (1920) Calciumhydroxid als Mittel zum Behandeln und Füllen von Wurzelkanälen. Med. Diss Würzburg 53.
33. Taşdemir T, Aydemir H, Inan U et al. (2005) Canal preparation with Hero 642 rotary Ni-Ti instruments compared with stainless steel hand K-file assessed using computed tomography. Int Endod J 38:402-408
34. Torabinejad M, Handysides R, Khademi AA et al. (2002) Clinical implications of the smear layer in endodontics: a review. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 94:658-666
35. Grossman L (1982) Obturation of root canal. Endodontic Practice, vol 297. Lea & Febiger, Philadelphia 46. Guelzow A, Stamm O, Martus P et al. (2005) Comparative study of six rotary nickel-titanium systems and hand instrumentation for root canal preparation. Int Endod J 38:743-752
36. Ramezanali F, Afkhami F, Soleimani A et al. (2015) Comparison of Cleaning Efficacy and Instrumentation Time in Primary Molars: Mtwo Rotary Instruments vs. Hand K-Files. Iran Endod J 10:240-243
37. Azar MR, Safi L, Nikaein A (2012) Comparison of the cleaning capacity of Mtwo and Pro Taper rotary systems and manual instruments in primary teeth. Dent Res J (Isfahan) 9:146-151
38. Taha NA, Ozawa T, Messer HH (2010) Comparison of three techniques for preparing oval-shaped root canals. J Endod 36:532-535
39. Söderholm KJ (1991) Correlation of in vivo and in vitro performance of adhesive restorative materials: a report of the ASC MD156 Task Group on Test Methods for the Adhesion of Restorative Materials. Dent Mater 7:74-83
40. Kim YK, Grandini S, Ames JM et al. (2010) Critical review on methacrylate resin-based root canal sealers. J Endod 36:383-399
41. Eldeniz AU, Mustafa K, Ørstavik D et al. (2007) Cytotoxicity of new resin-, calcium hydroxide-and silicone-based root canal sealers on fibroblasts derived from human gingiva and L929 cell lines. Int Endod J 40:329-337
42. Rolland SL, Carrick TE, Walls AW et al. (2007) Dentin decontamination using chloramine T prior to experiments involving bacteria. Dent Mater 23:1468- 1472
43. Scherrer SS, Cesar PF, Swain MV (2010) Direct comparison of the bond strength results of the different test methods: a critical literature review. Dent Mater 26:e78-93
44. Pintor AV, Dos Santos MR, Ferreira DM et al. (2016) Does Smear Layer Removal Influence Root Canal Therapy Outcome? A Systematic Review. J Clin Pediatr Dent 40:1-7
45. Di Lenarda R, Cadenaro M, Sbaizero O (2000) Effectiveness of 1 mol L -1 citric acid and 15% EDTA irrigation on smear layer removal. Int Endod J 33:46-52
46. Prado M, Simão RA, Gomes BP (2013) Effect of different irrigation protocols on resin sealer bond strength to dentin. J Endod 39:689-692
47. de Assis DF, do Prado M, Simão RA (2012) Effect of disinfection solutions on the adhesion force of root canal filling materials. J Endod 38:853-855
48. Erdemir A, Ari H, Güngüneş H et al. (2004) Effect of medications for root canal treatment on bonding to root canal dentin. J Endod 30:113-116
49. Gaddala N, Veeramachineni C, Tummala M (2015) Effect of Peracetic Acid as A Final Rinse on Push Out Bond Strength of Root Canal Sealers to Root Dentin. J Clin Diagn Res 9:ZC75-77
50. Mobarak EH, El-Badrawy W, Pashley DH et al. (2010) Effect of pretest storage conditions of extracted teeth on their dentin bond strengths. J Prosthet Dent 104:92-97
51. Galvan DA, Ciarlone AE, Pashley DH et al. (1994) Effect of smear layer removal on the diffusion permeability of human roots. J Endod 20:83-86
52. Marley JT, Ferguson DB, Hartwell GR (2001) Effects of chlorhexidine gluconate as an endodontic irrigant on the apical seal: short-term results. J Endod 27:775-778
53. Stavileci M, Hoxha V, Görduysus Ö et al. (2013) Effects of preparation techniques on root canal shaping assessed by micro-computed tomography. Med Sci Monit Basic Res 19:163-168
54. Lertchirakarn V, Timyam A, Messer HH (2002) Effects of root canal sealers on vertical root fracture resistance of endodontically treated teeth. J Endod 28:217- 219
55. Mitchem JC, Gronas DG (1986) Effects of time after extraction and depth of dentin on resin dentin adhesives. J Am Dent Assoc 113:285-287
56. Schäfer E, Lohmann D (2002) Efficiency of rotary nickel-titanium FlexMaster instruments compared with stainless steel hand K-Flexofile--Part 1. Shaping ability in simulated curved canals. Int Endod J 35:505-513
57. Schäfer E, Lohmann D (2002) Efficiency of rotary nickel-titanium FlexMaster instruments compared with stainless steel hand K-Flexofile--Part 2. Cleaning effectiveness and instrumentation results in severely curved root canals of extracted teeth. Int Endod J 35:514-521
58. Hellwig E, Klimek J, Attin T (2009) Einführung in die Zahnerhaltung. Deutscher Zahnärzte Verlag
59. Heidemann D (2001) Endodontie -Praxis der Zahnheilkunde. Urban & Fischer München, 4. Auflage, 1-24, 79-122.
60. Mokhtari H, Shahi S, Janani M et al. (2015) Evaluation of apical leakage in root canals obturated with three different sealers in presence or absence of smear layer. Iran Endod J 10:131-134
61. Sevimay S, Kalayci A (2005) Evaluation of apical sealing ability and adaptation to dentine of two resin-based sealers. J Oral Rehabil 32:105-110
62. Konjhodzic-Prcic A, Jakupovic S, Hasic-Brankovic L et al. (2015) Evaluation of Biocompatibility of Root Canal Sealers on L929 Fibroblasts with Multiscan EX Spectrophotometer. Acta Inform Med 23:135-137
63. Mutoh N, Satoh T, Watabe H et al. (2013) Evaluation of the biocompatibility of resin-based root canal sealers in rat periapical tissue. Dent Mater J 32:413- 419
64. Yilmaz Z, Dogan AL, Özdemir O et al. (2012) Evaluation of the cytotoxicity of different root canal sealers on L929 cell line by MTT assay. Dent Mater J 31:1028-1032
65. Cruz-Filho AM, Sousa-Neto MD, Saquy PC et al. (2001) Evaluation of the effect of EDTAC, CDTA, and EGTA on radicular dentin microhardness. J Endod 27:183-184
66. De-Deus G, Paciornik S, Mauricio MH (2006) Evaluation of the effect of EDTA, EDTAC and citric acid on the microhardness of root dentine. Int Endod J 39:401-407
67. Ari H, Erdemir A, Belli S (2004) Evaluation of the effect of endodontic irrigation solutions on the microhardness and the roughness of root canal dentin. J Endod 30:792-795
68. Huang TH, Lee H, Kao CT (2001) Evaluation of the genotoxicity of zinc oxide eugenol-based, calcium hydroxide-based, and epoxy resin-based root canal sealers by comet assay. J Endod 27:744-748
69. Mai S, Kim YK, Hiraishi N et al. (2009) Evaluation of the true self-etching potential of a fourth generation self-adhesive methacrylate resin-based sealer. J Endod 35:870-874
70. Baumann A, Beer R (2007) Farbatlanten der Zahnmedizin -Endodontologie. Georg Thieme Verlag KG Stuttgart
71. DGZMK (2005) Gemeinsame Stellungnahme der DGZMK und DGZ zum Thema Maschinelle Wurzelkanalaufbereitung. DZZ 60 26. DGZMK (2006) Gemeinsame Stellungnahme der DGZMK und der DGZ zum Thema Wurzelkanalspülung. DZZ 10
72. Hülsmann M, Schäfer E, Petschelt A et al. (2004) Good clinical practice: Die Wurzelkanalbehandlung. DZZ 60 (2005) 8
73. Rödig T, Hülsmann M, Nordmeyer S et al. (2009) Grundlagen der modernen Endodontie. Spitta Verlag, Balingen
74. Pashley D, Walton R (1994) Histology and physiology of the dental pulp. Endodontics. 320-354
75. da Silva LM, Andrade AM, Machuca MF et al. (2008) Influence of different adhesive systems on the pull-out bond strength of glass fiber posts. J Appl Oral Sci 16:232-235
76. Plotino G, Grande NM, Tocci L et al. (2014) Influence of Different Apical Preparations on Root Canal Cleanliness in Human Molars: a SEM Study. J Oral Maxillofac Res 5:e4
77. Roggendorf MJ, Ebert J, Petschelt A et al. (2007) Influence of moisture on the apical seal of root canal fillings with five different types of sealer. J Endod 33:31-33
78. Gesi A, Raffaelli O, Goracci C et al. (2005) Interfacial strength of Resilon and gutta-percha to intraradicular dentin. J Endod 31:809-813
79. Sipert CR, Hussne RP, Nishiyama CK et al. (2005) In vitro antimicrobial activity of Fill Canal, Sealapex, Mineral Trioxide Aggregate, Portland cement and EndoRez. Int Endod J 38:539-543
80. Brzovic V, Miletic I, Zeljezic D et al. (2009) In vitro genotoxicity of root canal sealers. Int Endod J 42:253-263
81. Boutsioukis C, Lambrianidis T, Kastrinakis E (2009) Irrigant flow within a prepared root canal using various flow rates: a Computational Fluid Dynamics study. Int Endod J 42:144-155
82. Goracci C, Grandini S, Bossù M et al. (2007) Laboratory assessment of the retentive potential of adhesive posts: a review. J Dent 35:827-835
83. Shemesh H, Wu MK, Wesselink PR (2006) Leakage along apical root fillings with and without smear layer using two different leakage models: a two-month longitudinal ex vivo study. Int Endod J 39:968-976
84. Wu MK, De Gee AJ, Wesselink PR (1994) Leakage of four root canal sealers at different thickness. Int Endod J 27:304-308
85. Sleder FS, Ludlow MO, Bohacek JR (1991) Long-term sealing ability of a calcium hydroxide sealer. J Endod 17:541-543
86. Arnitz R, Nagl M, Gottardi W (2009) Microbicidal activity of monochloramine and chloramine T compared. J Hosp Infect 73:164-170
87. Tay FR, Pashley DH (2007) Monoblocks in root canals: a hypothetical or a tangible goal. J Endod 33:391-398
88. Tronstad L, Andreasen JO, Hasselgren G et al. (1981) pH changes in dental tissues after root canal filling with calcium hydroxide. J Endod 7:17-21
89. De-Deus G, Coutinho-Filho T, Reis C et al. (2006) Polymicrobial leakage of four root canal sealers at two different thicknesses. J Endod 32:998-1001
90. Tepel J, Schäfer E, Hoppe W (1997) Properties of endodontic hand instruments used in rotary motion. Part 3. Resistance to bending and fracture. J Endod 23:141-145
91. Khoroushi M, Kachuei M (2014) Pull-out bond strength of a self-adhesive resin cement to NaOCl-treated root dentin: effect of antioxidizing agents. Restor Dent Endod 39:95-103
92. Leonardo MR, Leal JM, Simões Filho AP (1980) Pulpectomy: immediate root canal filling with calcium hydroxide. Concept and procedures. Oral Surg Oral Med Oral Pathol 49:441-450
93. Roydhouse RH (1970) Punch-shear test for dental purposes. J Dent Res 49:131-136
94. Costa JA, Rached-Júnior FA, Souza-Gabriel AE et al. (2010) Push-out strength of methacrylate resin-based sealers to root canal walls. Int Endod J 43:698-706
95. Endodontology ESE (2006) Quality guidelines for endodontic treatment: consensus report of the European Society of Endodontology. Int Endod J 39:921-930
96. Rôças IN, Lima KC, Siqueira JF (2013) Reduction in bacterial counts in infected root canals after rotary or hand nickel-titanium instrumentation--a clinical study. Int Endod J 46:681-687
97. Reeh ES, Messer HH, Douglas WH (1989) Reduction in tooth stiffness as a result of endodontic and restorative procedures. J Endod 15:512-516
98. McGurkin-Smith R, Trope M, Caplan D et al. (2005) Reduction of intracanal bacteria using GT rotary instrumentation, 5.25% NaOCl, EDTA, and Ca(OH) 2 . J Endod 31:359-363
99. Sano H, Shono T, Sonoda H et al. (1994) Relationship between surface area for adhesion and tensile bond strength--evaluation of a micro-tensile bond test. Dent Mater 10:236-240
100. Dickens SH, Milos MF (2002) Relationship of dentin shear bond strengths to different laboratory test designs. Am J Dent 15:185-192
101. Yamaguchi M, Yoshida K, Suzuki R et al. (1996) Root canal irrigation with citric acid solution. J Endod 22:27-29
102. Peters OA, Boessler C, Paqué F (2010) Root canal preparation with a novel nickel-titanium instrument evaluated with micro-computed tomography: canal surface preparation over time. J Endod 36:1068-1072
103. Azar MR, Mokhtare M (2011) Rotary Mtwo system versus manual K-file instruments: efficacy in preparing primary and permanent molar root canals. Indian J Dent Res 22:363
104. Mader CL, Baumgartner JC, Peters DD (1984) Scanning electron microscopic investigation of the smeared layer on root canal walls. J Endod 10:477-483
105. Salz U, Poppe D, Sbicego S et al. (2009) Sealing properties of a new root canal sealer. Int Endod J 42:1084-1089
106. Fróes JA, Horta HG, da Silveira AB (2000) Smear layer influence on the apical seal of four different obturation techniques. J Endod 26:351-354
107. Brännström M (1984) Smear layer: pathological and treatment considerations. Oper Dent Suppl 3:35-42
108. Zehnder M, Lehnert B, Schönenberger K et al. (2003) Spüllösungen und medikamentöse Einlagen in der Endodontie. Schweizerische Monatsschrift für Zahnmedizin 01/2003; 113
109. DGZMK (1999) Stellungnahme Wurzelkanalfüllpasten und -füllstifte. DZZ 24. DGZMK (2001) Stellungnahme zum Thema Wurzelkanalaufbereitungen.
110. Jaffer S, Oesterle LJ, Newman SM (2009) Storage media effect on bond strength of orthodontic brackets. Am J Orthod Dentofacial Orthop 136:83-86
111. Machian GR, Peters DD, Lorton L (1982) The comparative efficiency of four types of endodontic instruments. J Endod 8:398-402
112. Marshall GW, Marshall SJ, Kinney JH et al. (1997) The dentin substrate: structure and properties related to bonding. J Dent 25:441-458
113. Torneck CD, Moe H, Howley TP (1983) The effect of calcium hydroxide on porcine pulp fibroblasts in vitro. J Endod 9:131-136
114. Gängler P, Hoffmann T (2010) Konservierende Zahnheilkunde und Parodontologie. Georg Thieme Verlag, Stuttgart 48. Hashem AA, Ghoneim AG, Lutfy RA et al. (2009) The effect of different irrigating solutions on bond strength of two root canal-filling systems. J Endod 35:537-540
115. Said HM, Bakar WZ, Farea M et al. (2012) The effect of different sealer placement techniques on sealing Ability: An in vitro study. J Conserv Dent 15:257-260
116. Sayin TC, Serper A, Cehreli ZC et al. (2007) The effect of EDTA, EGTA, EDTAC, and tetracycline-HCl with and without subsequent NaOCl treatment on the microhardness of root canal dentin. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 104:418-424
117. Belli S, Erdemir A, Ozcopur M et al. (2005) The effect of fibre insertion on fracture resistance of root filled molar teeth with MOD preparations restored with composite. Int Endod J 38:73-80
118. D'Arcangelo C, Cinelli M, De Angelis F et al. (2007) The effect of resin cement film thickness on the pullout strength of a fiber-reinforced post system. J Prosthet Dent 98:193-198
119. von Fraunhofer JA, Branstetter J (1982) The physical properties of four endodontic sealer cements. J Endod 8:126-130
120. Mickel AK, Chogle S, Liddle J et al. (2007) The role of apical size determination and enlargement in the reduction of intracanal bacteria. J Endod 33:21-23
121. George S, Kishen A, Song KP (2005) The role of environmental changes on monospecies biofilm formation on root canal wall by Enterococcus faecalis. J Endod 31:867-872
122. Lee JJ, Nettey-Marbell A, Cook A et al. (2007) Using extracted teeth for research: the effect of storage medium and sterilization on dentin bond strengths. J Am Dent Assoc 138:1599-1603
123. Pawlicka H (1982) Verwendung der Chelatverbindungen zur Erweiterung der Wurzelkanäle: Mikrohärteuntersuchung. Stomatol DDR 32:355-361
124. Orstavik D (1983) Weight loss of endodontic sealers, cements and pastes in water. Scand J Dent Res 91:316-319
125. Wilson AD, Clinton DJ, Miller RP (1973) Zinc oxide-eugenol cements. IV. Microstructure and hydrolysis. J Dent Res 52:253-260

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