Dokument
Titel: | Crosslinking studies of the bacterial RNase P holoenzyme and functional characterisation of the novel protein-only RNase P from Arabidopsis thaliana |
Autor: | Pavlova, Liudmila Viktorovna |
Weitere Beteiligte: | Hartmann, Roland (Prof. Dr.) |
Veröffentlicht: | 2012 |
URI: | https://archiv.ub.uni-marburg.de/diss/z2012/0778 |
DOI: | https://doi.org/10.17192/z2012.0778 |
URN: | urn:nbn:de:hebis:04-z2012-07782 |
DDC: | Naturwissenschaften |
Titel (trans.): | Crosslinking-Studien an bakteriellem RNase P Holoenzym und funktionelle Untersuchungen der kürzlich entdeckten Protein-Enzyme der RNase P von Arabidopsis thaliana |
Publikationsdatum: | 2012-09-14 |
Lizenz: | https://rightsstatements.org/vocab/InC-NC/1.0/ |
Schlagwörter: |
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Kinetics, Crosslinking, Proteinaceous RNase P, Catalytical mechanism, Endoribonuklease P |
Summary:
Ribonuclease P (RNase P) is the ubiquitous processing enzyme that generates the mature 5'-termini of tRNA molecules. In Bacteria and Archaea the enzyme is a ribonucleoprotein, whereas in some Eukarya the RNAse P is exclusively proteinaceous. This work is focused on two topics: (i) crosslinking studies of bacterial RNase P holoenzyme utilizing novel 2’- ribooligonucleotides and (ii) functional investigations to the catalytical mechanism of recently discovered protein-only RNase P from Arabidopsis thaliana.
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