Publikationsserver der Universitätsbibliothek Marburg
Titel:Energiestoffwechsel von Cytochrom-freien methanogenen Archaea bei Wachstum auf H2 und CO2
Autor:Kaster, Anne-Kristin
Weitere Beteiligte: Thauer, Rudolf Kurt (Prof.)
Veröffentlicht:2011
URI:https://archiv.ub.uni-marburg.de/diss/z2011/0063
DOI: https://doi.org/10.17192/z2011.0063
URN: urn:nbn:de:hebis:04-z2011-00639
DDC: Biowissenschaften, Biologie
Titel (trans.):Energy conservation of cytochrome-free methanogens growing on H2 and CO2
Publikationsdatum:2011-02-18
Lizenz:https://rightsstatements.org/vocab/InC-NC/1.0/

Dokument

Schlagwörter:
Methanosarcina barkeri, Metronidazol, Methanothermobacter marburgensis, H2-Schwellenkonzentration, Ferredoxin, Wachstumsertrag, Methanothermobacter marburgensis, Growth yield, H2-threshold, Ferredoxin

Zusammenfassung:
Methanogene Archaea sind Organismen, die Methan als Endprodukt ihres anaeroben Energiestoffwechsels bilden. Es gibt zwei stoffwechselphysiologisch unterschiedliche Gruppen: Zum einen die Cytochrom-freien Methanogenen, welche nur H2 und CO2 und/oder Formiat zu Methan umsetzen und zum anderen die Cytochrom-haltigen Methanogenen, die auf Acetat, Methanol und/oder Methylamin spezialisiert sind und von denen nur einige wenige auf H2 und CO2 wachsen können. Da lediglich der Energiestoffwechsel Cytochrom-haltiger Methanogener bei Wachstum auf H2 und CO2 weitgehend verstanden ist, war es das Ziel der vorliegenden Arbeit, den Energiestoffwechsel Cytochrom-freier Methanogener aufzuklären. Dazu wurden zunächst die Wachstumserträge und H2-Schwellenkonzentrationen von zwei Cytochrom-freien Methanogenen bestimmt und mit denen eines Cytochrom-haltigen verglichen. Anschließend wurde die Kopplung des ersten und letzten Schrittes der Methanbildung aus H2 und CO2 auf Enzymebene untersucht.

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