Towards Bioorthogonal Enzymatic Uncaging Reactions

Towards Bioorthogonal Enzymatic Uncaging Reactions

In this thesis, engineered cytochrome P450 monooxygenase mutants are reported as highly active and selective catalysts for the bioorthogonal uncaging of diferent ether protected substrates, including the successful transfer of the uncaging reaction into living bacteria. This proof-of-principle study...

Fuld beskrivelse

Gespeichert in:
Bibliografiske detaljer
Hovedforfatter: Ritter, Cornelia
Andre forfattere: Meggers, Eric (Prof. Dr.) (BetreuerIn (Doktorarbeit))
Format: Dissertation
Sprog:engelsk
Udgivet: Philipps-Universität Marburg 2016
Fag:
caging
Enzym
Schutzgruppe
Chemische Biologie
P450
Aktivierung
Biowissenschaften, Biologie
Bioorthogonal
enzyme
chemical biology
activation
Life sciences
Online adgang:PDF-Volltext
Tags: Tilføj Tag
Ingen Tags, Vær først til at tagge denne postø!
Beskrivelse
Summary:In this thesis, engineered cytochrome P450 monooxygenase mutants are reported as highly active and selective catalysts for the bioorthogonal uncaging of diferent ether protected substrates, including the successful transfer of the uncaging reaction into living bacteria. This proof-of-principle study points towards the utility of bioorthogonal enzyme/protecting group pairs for applications in the life sciences.
DOI:10.17192/z2016.0051

Lignende værker