Purification and Functional Characterization of Recombinant Human ADAM8 protease

From recent studies in different tumor entities it became apparent that the membrane-anchored metalloprotease-disintegrin ADAM8 plays an active role in tumor progression and consequently in patient prognosis. It is therefore desirable to understand the functional role of ADAM8 in vitro by characteri...

Ausführliche Beschreibung

Gespeichert in:
1. Verfasser: Xiangdi Yu
Beteiligte: Bartsch, Jörg W. (Prof. Dr.) (BetreuerIn (Doktorarbeit))
Format: Dissertation
Veröffentlicht: Philipps-Universität Marburg 2015
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1. Anderson IC, Shipp M, Docherty AJP, Teicher BA (1996) Combination therapy including a gelatinase inhibitor and cytotoxic agent reduces local invasion and metastasis of murine Lewis lung carcinoma. Cancer Res 56: 715-718.

2. Duhamel S, Hebert J, Gaboury L, Bouchard A, Simon R, Sauter G (2012) Sef downregulation by Ras causes MEK1/2 to become aberrantly nuclear localized leading to polyploidy and neoplastic transformation. Cancer Res 72: 626-35.

3. Brakebusch C, Fassler R (2005) Beta1 integrin function in vivo: adhesion, migration and more. Cancer Metastasis Rev 24: 403-411.

4. Zhang R, Yuan Y, Zuo J, Liu W (2012) Prognostic and clinical implication of a disintegrin and metalloprotease8 expression in pediatric medulloblastoma. J Neurol Sci 323: 46-51.

5. Henningsson F, Ding Z, Dahlin JS, Linkevicius M, Carlsson F, Grönvik KO, Hallgren J, Heyman B (2011) IgE-mediated enhancement of CD4+ T cell responses in mice requires antigen presentation by CD11c+ cells and not by B cells. PLoS ONE 6: e2176.

6. Ruoslahti E (1995) Fibronectin and its alpha5 beta1 integrin receptor in malignancy. Invasion Metastasis 14: 87-97.

7. Gomez-Gaviro M, Dominguez-Luis M, Canchado J, Calafat J, Janssen H, Lara-pezzi E, Fourie A, Tugores A, Valenzuela-Fernandez A, Mollinedo F, Sanchez-Madrid F, Diaz-Gonzalez F (2007) Expression and regulation of the metalloproteinase ADAM-8 during human neutrophil pathophysiological activation and its catalytic activity on L-selectin shedding. J. Immunol. 178: References 48 8053-8063.

8. Leiss M, Beckmann K, Girós A, Costell M, Fä ssler R (2008) The role of integrin binding sites in fibronectin matrix assembly in vivo. Curr Opin Cell Biol. 20: 502-7.

9. Pankov R, Yamada KM (2002) Fibronectin at a glance. Journal of Cell Science 115: 3861-3. References 49

10. Anderson DG, Li X, Balian G (2005) A fibronectin fragment alters the metabolism by rabbit intervertevral disc cells in vitro. Spine 30: 1242-6.

11. Kijimoto-Ochiai S (2002) CD23 (the low-affinity IgE receptor) as a C-type lectin: a multidomain and multifunctional molecule. Cellular and Molecular Life Sciences 59: 648-664.

12. Ghayouri M, Boulware D, Nasir A, Strosberg J, Kvols L, Coppola D (2010) Activation of the serine/theronine protein kinase Akt in enteropancreatic neuroendocrine tumors. Anticancer Res 30: 5063-5067.

13. Fourie AM, Coles F, Moreno V, Karlsson L (2003) Catalytic activity of ADAM8, ADAM15, and MDC-L (ADAM28) on synthetic peptide substrates and in ectodomain cleavage of CD23. J Biol Chem 278: 30469-30477.

14. Lichtman AH, Abbas AK (2003) Cellular and molecular immunology. Philadelphia: Saunders. pp: 324-325.

15. Naus S, Reipschlä ger S, Wildeboer D, Lichtenthaler SF, Mitterreiter S, Guan Z, Moss ML, Bartsch JW (2006) Identification of candidate substrate for ectodomain shedding by the metalloprotease-disintegrin ADAM8.J Biol Chem 387: 337-346.

16. Stöcker W, Bode W (1995) Structural features of a superfamily of zinc-endopeptidases: the metzincins. Curr Opin Struct Biol 5: 383-90.

17. Inoki K, Li Y, Zhu T, Wu J, Guan KL (2002) TSC2 is phosphorylated and inhibited by Akt and suppresses mTOR signaling. Nat. Cell Biol 4: 648-657.

18. T, Isshiki A, Erickson HP, Fassler R (2001) Plasma fibronectin supports neuronal survival and reduces brain injury following transient focal cerebral ischemia but is not essential for skin-wound healing and hemostasis. Nat. Med 7: 324-330.

19. Zack MD, Melton MA, Stock JL, Storer CE, Barve RA, Minnerly JC,Weiss DJ, Stejskal JA, Tortorella MD, Turk JR, Shevlin KM, Malfait AM (2009) Reduced incidence and severity of experimental autoimmune arthritis in mice expressing catalytically inactive A distingrin and metalloprotease 8 (ADAM8).

20. Delespesse G, Sarfati M, Wu CY, Fournier S, Letellier M (1992)The low-affinity receptor for IgE. Immunol 125: 77-97.

21. Amour A, Knight CG, English WR, Webster A, Slocombe PM, Knä uper V, Docherty AJ, Becherer JD, Blobel CP, Murphy G (2002) The enzymatic activity of ADAM8 and ADAM9 is not regulated by TIMPs. FEBS Lett. 524: 154-158.

22. Hoekstra R, Eskens FA, Verweij J (2001) Matrix metalloproteinase inhibitors: current developments and future perspectives. Oncologist 6: 415-27.

23. Thurnher D (2012) ADAM8 in squamous cell carcinoma of the head and neck: a retrospective study. BMC Cancer12: 76.

24. Corbett SA, Lee L, Wilson CL, Schwarzbauer JE (1997) Covalent cross-linking of fibronectin to fibrin is required for maximal cell adhesion to a fibronectin-fibrin matrix. J Biol Chem 272: 2499.

25. Buzza MS, Zamurs L, Sun J, Bird CH, Smith AI, Trapani JA, Froelich CJ, Nice EC, Bird PI (2005) Extracellular matrix remodeling by human granzyme B via cleavage of vitronectin, fibronectin, and lamin. J.Biol.Chem 280: 23549-23558.

26. Bartsch JW, Wildeboer D, Koller G, Naus S, Rittger A, Moss ML, Minai Y, Jockusch H (2010) Tumor Necrosis Factor-α (TNF-α) regulates shedding of TNF-a receptor 1 by the Metallopprotease-Disintegrin ADAM8: Evidence for a proteaseregulated feedback loop in neuroprotection. J. Neurosci. 30:12210-12218.

27. Wheeler DJ, Parveen S, Pollock K, Williams RJ (1998) Inhibition of sCD23 and immunoglobulin E release from human B cells by a metalloproteinase inhibitor, GI 129471.Immunology 95: 105-110.

28. Lloyd AC (2006) Distinct functions for ERKs? Journal of Biology 5:13.

29. Schwinn MK, Peters DM (2009) Functional properties of fibronectin in the trabecular meshwork. Exp Eye Res 88: 689-93.

30. Fibronectin expression modulates mammary epithelial cell proliferation during acinar differentiation. Cancer Research 68: 3185-92.

31. Ruel N, Markova DZ, Adams SL, Scanzello C, Cs-Szabo G, Gerard D, Shi P, Anderson G, Zack M, An HS, Chen D, Zhang YJ (2014) Fibronectin fragments and the cleaving enzyme ADAM-8 in the degenerative human intervertebral disc. Spine 39: 1274-9.

32. Bonnefoy JY, Plater-Zyberk C, Lecoanet-Henchoz S, Gauchat JF, Aubry JP, Graber P (1996) A new role for CD23 in inflammation. Immunol.17: 418-420.

33. Moss ML, Rasmussen FH (2007) Fluorescent substrates for the proteinases ADAM17, ADAM10, ADAM8, and ADAM12 useful for high-throughput inhibitor screening. Anal Biochem 366: 144-8.

34. Henry C, Hsia, Mohan R, Siobhan A, Corbett (2014) The fate of internalized α5 integrin is regulated by matrix-capable fibronectin. Journal of surgical research 191: 268-79.

35. Nishimura D, Sakai H, Sato F, Nishimura S, Toyama-Sorimachi N, Bartsch JW (2015) Sehara-Fujisawa A. Roles of ADAM8 in elimination of injured muscle fibers prior to skeletal muscle regeneration. Mech Dev. 135: 58-67.

36. Weskamp G, Ford JW, Sturgill J, Martin S, Docherty AJ, Blobel CP (2006) ADAM10 is a principal 'sheddase' of the low-affinity immunoglobulin E receptor Nat. Immunol. 7: 1293-1298.