In der Spermatogenese von Drosophila melanogaster wird das Chromatin-assoziierte Protein Mst77F in seiner Translation, in seiner Kernlokalisation und in seiner Chromatin-kompaktierenden Funktion über distinkte Bereiche kontrolliert.

In der Spermiogenese von Drosophila wird im Kanustadium der Wechsel von einer auf Histonen- zu einer auf Protaminen basierenden Chromatinstruktur von einer großen Menge an DNA Brüchen begleitet. Die Analyse der Induktion wie auch der Reparatur dieser Brüche, liegt im Fokus des ersten Teils dieser Di...

Ausführliche Beschreibung

Gespeichert in:
1. Verfasser: Kaiser, Sophie
Beteiligte: Renkawitz-Pohl, Renate (Prof. Dr.) (BetreuerIn (Doktorarbeit))
Format: Dissertation
Sprache:Deutsch
Veröffentlicht: Philipps-Universität Marburg 2014
Biologie
Ausgabe:http://dx.doi.org/10.17192/z2014.0377
Schlagworte:
Online Zugang:PDF-Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
last_indexed 2015-10-06T23:59:59Z
author Kaiser, Sophie
spellingShingle Kaiser, Sophie
Mst77F
Spermatogenese
Chromatin
Drosophila
Mst77F
Biowissenschaften, Biologie
In der Spermatogenese von Drosophila melanogaster wird das Chromatin-assoziierte Protein Mst77F in seiner Translation, in seiner Kernlokalisation und in seiner Chromatin-kompaktierenden Funktion über distinkte Bereiche kontrolliert.
During Drosophila spermatogenesis the replacement of histones by protamines at the canoe stage is accompanied by numerous DNA breaks. The induction and repair of these DNA breaks are analyzed in the first part of this thesis. First I analyzed the influence of endonucleases in setting DNA breaks, but neither Tengl1-4, nor Squash or Squash-like are expressed during the switch and therefore are not required for the induction of DNA breaks. In parallel to the DNA breaks high amounts of UbcD6, the Drosophila homolog of yeast Rad6, are detectable. A specific role of the enzyme in DNA repair can be considered. But unfortunately it is not possible to express the fusion protein UbcD6 eGFP in canoe stage nuclei. Therefore we started analysing other components of the Rad6 postreplication repair. However, the transcripts of the components are transcribed only in early stages of spermatogenesis. Thus, the repair of DNA breaks via UbcD6 seems to base on an alternative repair mechanism. The major part of this thesis deals with the linker histone-like protein Mst77F, a chromatin component of mature sperm. It was shown that the transcription of Mst77F depends on tTAFs und that Mst77F mRNA underlies translational repression. In addition, I could show that, in contrast to the majority of testis-specific genes, the 5' UTR is not sufficient to mediate translational repression of the corresponding mRNAs. Interestingly, analyses of transgenic flies clearly highlight distinct regions within the ORF essential for translational repression and activation of Mst77F mRNAs. Furthermore, analyzing ectopic expression of Mst77F suggests that translation of Mst77F may be specific for germ cells and that in spermatocytes Mst77F transcript expression is controlled by a special transcriptional machinery. Mst77F is a chromatin component of the mature sperm and surprisingly, after ectopic expression of Mst77F eGFP in a wild-type background, about 20 % of late spermatid nuclei look abnormal. It can be demonstrated that the phenotype depends on special domains in the protein structure, a C terminal domain with several nuclear location signals and one N terminal coiled coil domain. The coiled coil domain is a typical protein protein interaction domain which allows a kind of crosslinking of the proteins. This fits very well with in vitro data, indicating a role of Mst77F in compaction of the chromatin. We hypothesize that Mst77F affords, in case of sperm maturation, chromatin condensation in specific regions.
publisher Philipps-Universität Marburg
topic Mst77F
Spermatogenese
Chromatin
Drosophila
Mst77F
Biowissenschaften, Biologie
license_str https://creativecommons.org/licenses/by-nc-sa/4.0
oai_set_str_mv open_access
doc-type:doctoralThesis
ddc:570
xMetaDissPlus
title In der Spermatogenese von Drosophila melanogaster wird das Chromatin-assoziierte Protein Mst77F in seiner Translation, in seiner Kernlokalisation und in seiner Chromatin-kompaktierenden Funktion über distinkte Bereiche kontrolliert.
title_short In der Spermatogenese von Drosophila melanogaster wird das Chromatin-assoziierte Protein Mst77F in seiner Translation, in seiner Kernlokalisation und in seiner Chromatin-kompaktierenden Funktion über distinkte Bereiche kontrolliert.
title_full In der Spermatogenese von Drosophila melanogaster wird das Chromatin-assoziierte Protein Mst77F in seiner Translation, in seiner Kernlokalisation und in seiner Chromatin-kompaktierenden Funktion über distinkte Bereiche kontrolliert.
title_fullStr In der Spermatogenese von Drosophila melanogaster wird das Chromatin-assoziierte Protein Mst77F in seiner Translation, in seiner Kernlokalisation und in seiner Chromatin-kompaktierenden Funktion über distinkte Bereiche kontrolliert.
title_full_unstemmed In der Spermatogenese von Drosophila melanogaster wird das Chromatin-assoziierte Protein Mst77F in seiner Translation, in seiner Kernlokalisation und in seiner Chromatin-kompaktierenden Funktion über distinkte Bereiche kontrolliert.
title_sort In der Spermatogenese von Drosophila melanogaster wird das Chromatin-assoziierte Protein Mst77F in seiner Translation, in seiner Kernlokalisation und in seiner Chromatin-kompaktierenden Funktion über distinkte Bereiche kontrolliert.
doi_str_mv http://dx.doi.org/10.17192/z2014.0377
edition http://dx.doi.org/10.17192/z2014.0377
description In der Spermiogenese von Drosophila wird im Kanustadium der Wechsel von einer auf Histonen- zu einer auf Protaminen basierenden Chromatinstruktur von einer großen Menge an DNA Brüchen begleitet. Die Analyse der Induktion wie auch der Reparatur dieser Brüche, liegt im Fokus des ersten Teils dieser Dissertation. Hierfür wird zunächst die Beteiligung von Endonukleasen am Setzen der DNA Brüche untersucht, doch aufgrund der Expressionsmuster können die Nukleasen Tengl1-4, wie auch Squash und Squash like als Induktoren der Strangbrüche ausgeschlossen werden. Parallel zu den DNA Brüchen werden große Mengen an UbcD6, dem Drosophila Homolog zu Rad6 in Hefen, exprimiert und eine Beteiligung des Enzyms an der Reparatur der DNA wurde in Betracht gezogen. Jedoch ist es nicht möglich das Fusionsprotein UbcD6 eGFP im Kanustadium zu exprimieren und so wird die Verteilung weiterer Proteine der Rad6 Postreplikationsreparatur untersucht. Allerdings sind die Transkripte der Komponenten ausschließlich in frühen Spermatogenesestadien zu detektieren und so scheint die Reparatur der Brüche durch UbcD6 auf anderen Mechanismen zu beruhen. Der Schwerpunkt der vorliegenden Dissertation befasst sich jedoch mit der Analyse des Histon ähnlichen Proteins Mst77F. Mst77F ist ein Chromatin assoziiertes Protein, dessen Transkription tTAF abhängig erfolgt und zudem einer translationalen Repression unterliegt. Jedoch wird die translationale Repression von Mst77F, im Gegensatz zu den meisten anderen Testis spezifischen Genen, nicht über die 5' UTR reguliert. Vielmehr zeigen meine Analysen von transgenen Fliegen, dass die Repression wie auch die Aktivierung der Translation der Mst77F mRNA über Bereiche im ORF kontrolliert wird. Weiterführende Expressionsstudien zeigen zudem, dass Mst77F in Spermatozyten einer speziellen Transkriptionsrepressions¬kontrolle unterliegt und die Translation von Mst77F spezifisch in der Spermiogenese aktiviert wird. Eine Translation in somatischen Zellen erfolgt hingegen nur sehr ineffizient. Mst77F bildet eine Komponente des reifen Spermiums und überraschenderweise zeigen 20 % der späten Spermatidenkerne, nach einer ektopischen Expression von Mst77F eGFP, im wildtypischen Mst77F Hintergrund, eine abnormale Kernform. Zudem kann gezeigt werden, dass dieser Phänotyp in Abhängigkeit von speziellen Proteindomänen, einer C terminalen Domäne mit Kernlokalisationssignalen und einer N terminalen Coiled Coil Domäne, auftritt. Über die Coiled Coil Domäne können Proteininteraktionen bzw. Vernetzungen von Proteinen erfolgen. in vitro führt dies zu einer Kompaktierung im Chromatin, in Übereinstimmung mit den hier präsentierten in vivo Daten. Daher kann postuliert werden, dass Mst77F während der Reifung des Spermiums, eine Region spezifische Chromatinkondensation, ermöglicht.
publishDate 2014
era_facet 2014
language German
building Fachbereich Biologie
first_indexed 2015-09-10T00:00:00Z
contents During Drosophila spermatogenesis the replacement of histones by protamines at the canoe stage is accompanied by numerous DNA breaks. The induction and repair of these DNA breaks are analyzed in the first part of this thesis. First I analyzed the influence of endonucleases in setting DNA breaks, but neither Tengl1-4, nor Squash or Squash-like are expressed during the switch and therefore are not required for the induction of DNA breaks. In parallel to the DNA breaks high amounts of UbcD6, the Drosophila homolog of yeast Rad6, are detectable. A specific role of the enzyme in DNA repair can be considered. But unfortunately it is not possible to express the fusion protein UbcD6 eGFP in canoe stage nuclei. Therefore we started analysing other components of the Rad6 postreplication repair. However, the transcripts of the components are transcribed only in early stages of spermatogenesis. Thus, the repair of DNA breaks via UbcD6 seems to base on an alternative repair mechanism. The major part of this thesis deals with the linker histone-like protein Mst77F, a chromatin component of mature sperm. It was shown that the transcription of Mst77F depends on tTAFs und that Mst77F mRNA underlies translational repression. In addition, I could show that, in contrast to the majority of testis-specific genes, the 5' UTR is not sufficient to mediate translational repression of the corresponding mRNAs. Interestingly, analyses of transgenic flies clearly highlight distinct regions within the ORF essential for translational repression and activation of Mst77F mRNAs. Furthermore, analyzing ectopic expression of Mst77F suggests that translation of Mst77F may be specific for germ cells and that in spermatocytes Mst77F transcript expression is controlled by a special transcriptional machinery. Mst77F is a chromatin component of the mature sperm and surprisingly, after ectopic expression of Mst77F eGFP in a wild-type background, about 20 % of late spermatid nuclei look abnormal. It can be demonstrated that the phenotype depends on special domains in the protein structure, a C terminal domain with several nuclear location signals and one N terminal coiled coil domain. The coiled coil domain is a typical protein protein interaction domain which allows a kind of crosslinking of the proteins. This fits very well with in vitro data, indicating a role of Mst77F in compaction of the chromatin. We hypothesize that Mst77F affords, in case of sperm maturation, chromatin condensation in specific regions.
url http://archiv.ub.uni-marburg.de/diss/z2014/0377/pdf/dsk.pdf
format Dissertation
institution Biologie
title_alt In Drosophila spermatogenesis translation, nuclear localization and chromatin compaction ability of the chromatin-associated protein Mst77F are regulated through destinct regions
author2 Renkawitz-Pohl, Renate (Prof. Dr.)
author2_role ths
ref_str_mv references
dewey-raw 570
dewey-search 570
genre Life sciences
genre_facet Life sciences
topic_facet Biowissenschaften, Biologie
thumbnail http://archiv.ub.uni-marburg.de/diss/z2014/0377/cover.png
spelling diss/z2014/0377 2015-10-06 In der Spermatogenese von Drosophila melanogaster wird das Chromatin-assoziierte Protein Mst77F in seiner Translation, in seiner Kernlokalisation und in seiner Chromatin-kompaktierenden Funktion über distinkte Bereiche kontrolliert. http://dx.doi.org/10.17192/z2014.0377 In der Spermiogenese von Drosophila wird im Kanustadium der Wechsel von einer auf Histonen- zu einer auf Protaminen basierenden Chromatinstruktur von einer großen Menge an DNA Brüchen begleitet. Die Analyse der Induktion wie auch der Reparatur dieser Brüche, liegt im Fokus des ersten Teils dieser Dissertation. Hierfür wird zunächst die Beteiligung von Endonukleasen am Setzen der DNA Brüche untersucht, doch aufgrund der Expressionsmuster können die Nukleasen Tengl1-4, wie auch Squash und Squash like als Induktoren der Strangbrüche ausgeschlossen werden. Parallel zu den DNA Brüchen werden große Mengen an UbcD6, dem Drosophila Homolog zu Rad6 in Hefen, exprimiert und eine Beteiligung des Enzyms an der Reparatur der DNA wurde in Betracht gezogen. Jedoch ist es nicht möglich das Fusionsprotein UbcD6 eGFP im Kanustadium zu exprimieren und so wird die Verteilung weiterer Proteine der Rad6 Postreplikationsreparatur untersucht. Allerdings sind die Transkripte der Komponenten ausschließlich in frühen Spermatogenesestadien zu detektieren und so scheint die Reparatur der Brüche durch UbcD6 auf anderen Mechanismen zu beruhen. Der Schwerpunkt der vorliegenden Dissertation befasst sich jedoch mit der Analyse des Histon ähnlichen Proteins Mst77F. Mst77F ist ein Chromatin assoziiertes Protein, dessen Transkription tTAF abhängig erfolgt und zudem einer translationalen Repression unterliegt. Jedoch wird die translationale Repression von Mst77F, im Gegensatz zu den meisten anderen Testis spezifischen Genen, nicht über die 5' UTR reguliert. Vielmehr zeigen meine Analysen von transgenen Fliegen, dass die Repression wie auch die Aktivierung der Translation der Mst77F mRNA über Bereiche im ORF kontrolliert wird. Weiterführende Expressionsstudien zeigen zudem, dass Mst77F in Spermatozyten einer speziellen Transkriptionsrepressions¬kontrolle unterliegt und die Translation von Mst77F spezifisch in der Spermiogenese aktiviert wird. Eine Translation in somatischen Zellen erfolgt hingegen nur sehr ineffizient. Mst77F bildet eine Komponente des reifen Spermiums und überraschenderweise zeigen 20 % der späten Spermatidenkerne, nach einer ektopischen Expression von Mst77F eGFP, im wildtypischen Mst77F Hintergrund, eine abnormale Kernform. Zudem kann gezeigt werden, dass dieser Phänotyp in Abhängigkeit von speziellen Proteindomänen, einer C terminalen Domäne mit Kernlokalisationssignalen und einer N terminalen Coiled Coil Domäne, auftritt. Über die Coiled Coil Domäne können Proteininteraktionen bzw. Vernetzungen von Proteinen erfolgen. in vitro führt dies zu einer Kompaktierung im Chromatin, in Übereinstimmung mit den hier präsentierten in vivo Daten. Daher kann postuliert werden, dass Mst77F während der Reifung des Spermiums, eine Region spezifische Chromatinkondensation, ermöglicht. opus:5639 2014 2015-09-10 During Drosophila spermatogenesis the replacement of histones by protamines at the canoe stage is accompanied by numerous DNA breaks. The induction and repair of these DNA breaks are analyzed in the first part of this thesis. First I analyzed the influence of endonucleases in setting DNA breaks, but neither Tengl1-4, nor Squash or Squash-like are expressed during the switch and therefore are not required for the induction of DNA breaks. In parallel to the DNA breaks high amounts of UbcD6, the Drosophila homolog of yeast Rad6, are detectable. A specific role of the enzyme in DNA repair can be considered. But unfortunately it is not possible to express the fusion protein UbcD6 eGFP in canoe stage nuclei. Therefore we started analysing other components of the Rad6 postreplication repair. However, the transcripts of the components are transcribed only in early stages of spermatogenesis. Thus, the repair of DNA breaks via UbcD6 seems to base on an alternative repair mechanism. The major part of this thesis deals with the linker histone-like protein Mst77F, a chromatin component of mature sperm. It was shown that the transcription of Mst77F depends on tTAFs und that Mst77F mRNA underlies translational repression. In addition, I could show that, in contrast to the majority of testis-specific genes, the 5' UTR is not sufficient to mediate translational repression of the corresponding mRNAs. Interestingly, analyses of transgenic flies clearly highlight distinct regions within the ORF essential for translational repression and activation of Mst77F mRNAs. Furthermore, analyzing ectopic expression of Mst77F suggests that translation of Mst77F may be specific for germ cells and that in spermatocytes Mst77F transcript expression is controlled by a special transcriptional machinery. Mst77F is a chromatin component of the mature sperm and surprisingly, after ectopic expression of Mst77F eGFP in a wild-type background, about 20 % of late spermatid nuclei look abnormal. It can be demonstrated that the phenotype depends on special domains in the protein structure, a C terminal domain with several nuclear location signals and one N terminal coiled coil domain. The coiled coil domain is a typical protein protein interaction domain which allows a kind of crosslinking of the proteins. This fits very well with in vitro data, indicating a role of Mst77F in compaction of the chromatin. We hypothesize that Mst77F affords, in case of sperm maturation, chromatin condensation in specific regions. urn:nbn:de:hebis:04-z2014-03775 2014-07-25 In Drosophila spermatogenesis translation, nuclear localization and chromatin compaction ability of the chromatin-associated protein Mst77F are regulated through destinct regions Li, C.; Vagin, V.V.; Lee, S.; Xu, J.; Ma, S. et al. (2009) Collapse of germline piRNAs in the absence of Argonaute3 reveals somatic piRNAs in flies. Cell 137(3): 509-21. 2009 Collapse of germline piRNAs in the absence of Argonaute3 reveals somatic piRNAs in flies Wente, S.R.; Rout, M.P. (2010) The nuclear pore complex and nuclear transport. Cold Spring Harbor perspectives in biology 2(10): a000562. 2010 The nuclear pore complex and nuclear transport Schulz, R.A.; Miksch, J.L.; Xie, X.L.; Cornish, J.A.; Galewsky, S. (1990) Expression of the Drosophila gonadal gene: alternative promoters control the germ-line expression of monocistronic and bicistronic gene transcripts. Development (Cambridge, England) 108(4): 613-22. 1990 Expression of the Drosophila gonadal gene: alternative promoters control the germ-line expression of monocistronic and bicistronic gene transcripts Brand, A.H.; Perrimon, N. (1993) Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development (Cambridge, England) 118(2): 401-15. 1993 Targeted gene expression as a means of altering cell fates and generating dominant phenotypes Schneider, I. (1972) Cell lines derived from late embryonic stages of Drosophila melanogaster. Journal of embryology and experimental morphology 27(2): 353-65. 1972 Cell lines derived from late embryonic stages of Drosophila melanogaster Kempe, E.; Muhs, B.; Schafer, M. (1993) Gene regulation in Drosophila spermatogenesis: analysis of protein binding at the translational control element TCE. Developmental genetics 14(6): 449-59. 1993 Gene regulation in Drosophila spermatogenesis: analysis of protein binding at the translational control element TCE Schafer, M.; Nayernia, K.; Engel, W.; Schafer, U. (1995) Translational control in spermatogenesis. Developmental biology 172(2): 344-52. 1995 Translational control in spermatogenesis Steger, K. (2001) Haploid spermatids exhibit translationally repressed mRNAs. Anatomy and embryology 203(5): 323-34. 2001 Haploid spermatids exhibit translationally repressed mRNAs Schulz, R.A.; Xie, X.L.; Miksch, J.L. (1990) cis-acting sequences required for the germ line expression of the Drosophila gonadal gene. Developmental biology 140(2): 455-8. 1990 ) cis-acting sequences required for the germ line expression of the Drosophila gonadal gene Vinson, C. ; Acharya, A. ; Taparowsky, E. J. (2006) Deciphering B-ZIP transcription factor interactions in vitro and in vivo. Biochimica et biophysica acta 1759(1-2): 4-12. 2006 Deciphering B-ZIP transcription factor interactions in vitro and in vivo Rathke, C.; Baarends, W.M.; Awe, S.; Renkawitz-Pohl, R. (2014) Chromatin dynamics during spermiogenesis. Biochimica et biophysica acta 1839(3): 155-68. 2014 Chromatin dynamics during spermiogenesis Chen, D.; McKearin, D. (2003) Dpp signaling silences bam transcription directly to establish asymmetric divisions of germline stem cells. Current biology : CB 13(20): 1786-91. 2003 Dpp signaling silences bam transcription directly to establish asymmetric divisions of germline stem cells Rathke, C.; Barckmann, B.; Burkhard, S.; Jayaramaiah-Raja, S.; Roote, J. et al. (2010) Distinct functions of Mst77F and protamines in nuclear shaping and chromatin condensation during Drosophila spermiogenesis. European journal of cell biology 89(4): 326-38. 2010 Distinct functions of Mst77F and protamines in nuclear shaping and chromatin condensation during Drosophila spermiogenesis Cho, C.; Willis, W.D.; Goulding, E.H.; Jung-Ha, H.; Choi, Y. C. et al. (2001) Haploinsufficiency of protamine-1 or -2 causes infertility in mice. Nature genetics 28(1): 82-6. 2001 Haploinsufficiency of protamine-1 or -2 causes infertility in mice Prasad, R.; Singhal, R.K.; Srivastava, D.K.; Molina, J.T.; Tomkinson, A.E. et al. (1996) Specific interaction of DNA polymerase beta and DNA ligase I in a multiprotein base excision repair complex from bovine testis. The Journal of biological chemistry 271(27): 16000-7. 1996 Specific interaction of DNA polymerase beta and DNA ligase I in a multiprotein base excision repair complex from bovine testis Lindsley, D. & Tokuyasu, K.T. (1980) Spermatogenesis. In: Ashburner, M., Wright, T.R.F. (Eds.), Genetics and Biology of Drosophila. Vol. 2d. Academic Press, New York, USA, 225-294 1980 Spermatogenesis Le, S.Y.; Maizel, J.V.J. (1997) A common RNA structural motif involved in the internal initiation of translation of cellular mRNAs. Nucleic acids research 25(2): 362-69. 1997 A common RNA structural motif involved in the internal initiation of translation of cellular mRNAs Snel, B.; Lehmann, G.; Bork, P.; Huynen, M.A. (2000) STRING: a web-server to retrieve and display the repeatedly occurring neighbourhood of a gene. Nucleic acids research 28(18): 3442-4. 2000 STRING: a web-server to retrieve and display the repeatedly occurring neighbourhood of a gene Yepiskoposyan, H.; Egli, D.; Fergestad, T.; Selvaraj, A.; Treiber, C. et al. (2006) Transcriptome response to heavy metal stress in Drosophila reveals a new zinc transporter that confers resistance to zinc. Nucleic acids research 34(17): 4866-77. 2006 Transcriptome response to heavy metal stress in Drosophila reveals a new zinc transporter that confers resistance to zinc Kibbe, W.A. (2007) OligoCalc: an online oligonucleotide properties calculator. Nucleic acids research 35(Web Server issue): W43-6. 2007 OligoCalc: an online oligonucleotide properties calculator Tweedie, S.; Ashburner, M.; Falls, K.; Leyland, P.; McQuilton, P. et al. (2009) FlyBase: enhancing Drosophila Gene Ontology annotations. Nucleic acids research 37(Database issue): D555-9. 2009 FlyBase: enhancing Drosophila Gene Ontology annotations Zhao, J.; Klyne, G.; Benson, E.; Gudmannsdottir, E.; White-Cooper, H. et al. (2010) FlyTED: the Drosophila Testis Gene Expression Database. Nucleic acids research 38(Database issue): D710-5. 2010 FlyTED: the Drosophila Testis Gene Expression Database Letunic, I.; Doerks, T.; Bork, P. (2012) SMART 7: recent updates to the protein domain annotation resource. Nucleic acids research 40(Database issue): D302-5. 2012 SMART 7: recent updates to the protein domain annotation resource Kuchta, K.; Barszcz, D.; Grzesiuk, E.; Pomorski, P.; Krwawicz, J. (2012) DNAtraffic--a new database for systems biology of DNA dynamics during the cell life. Nucleic acids research 40(Database issue): D1235-40. 2012 DNAtraffic--a new database for systems biology of DNA dynamics during the cell life Leduc, F.; Maquennehan, V.; Nkoma, G.B.; Boissonneault, G. (2008) DNA damage response during chromatin remodeling in elongating spermatids of mice. Biology of reproduction 78(2): 324- 32. 2008 DNA damage response during chromatin remodeling in elongating spermatids of mice Jorgensen, E.M.; Garber, R.L. (1987) Function and misfunction of the two promoters of the Drosophila Antennapedia gene. Genes and development 1(6): 544-55. 1987 Function and misfunction of the two promoters of the Drosophila Antennapedia gene Riggleman, B.; Wieschaus, E.; Schedl, P. (1989) Molecular analysis of the armadillo locus: uniformly distributed transcripts and a protein with novel internal repeats are associated with a Drosophila segment polarity gene. Genes and development 3(1): 96-113. 1989 Molecular analysis of the armadillo locus: uniformly distributed transcripts and a protein with novel internal repeats are associated with a Drosophila segment polarity gene Braun, R.E.; Peschon, J.J.; Behringer, R.R.; Brinster, R.L.; Palmiter, R.D. (1989) Protamine 3'- untranslated sequences regulate temporal translational control and subcellular localization of growth hormone in spermatids of transgenic mice. Genes and development 3(6): 793-802. 1989 Protamine 3'- untranslated sequences regulate temporal translational control and subcellular localization of growth hormone in spermatids of transgenic mice Sassone-Corsi, P. (2002) Unique chromatin remodeling and transcriptional regulation in spermatogenesis. Science (New York, N.Y.) 296(5576): 2176-8. 2002 Unique chromatin remodeling and transcriptional regulation in spermatogenesis Cote, J.; Ruiz-Carrillo, A. (1993) Primers for mitochondrial DNA replication generated by endonuclease G. Science (New York, N.Y.) 261(5122): 765-9. 1993 Primers for mitochondrial DNA replication generated by endonuclease G Rathke, C.; Baarends, W.M.; Jayaramaiah-Raja, S.; Bartkuhn, M.; Renkawitz, R. et al. (2007) Transition from a nucleosome-based to a protamine-based chromatin configuration during spermiogenesis in Drosophila. Journal of cell science 120(Pt 9): 1689-700. 2007 Transition from a nucleosome-based to a protamine-based chromatin configuration during spermiogenesis in Drosophila Kohler, M.; Ansieau, S.; Prehn, S.; Leutz, A.; Haller, H. et al. (1997) Cloning of two novel human importin-alpha subunits and analysis of the expression pattern of the importin-alpha protein family. FEBS letters 417(1): 104-8. 1997 Cloning of two novel human importin-alpha subunits and analysis of the expression pattern of the importin-alpha protein family Chintapalli, V.R.; Wang, J.; Dow, J.A.T. (2007) Using FlyAtlas to identify better Drosophila melanogaster models of human disease. Nature genetics 39(6): 715-20. 2007 Using FlyAtlas to identify better Drosophila melanogaster models of human disease Rubin, G.M.; Spradling, A.C. (1982) Genetic transformation of Drosophila with transposable element vectors. Science (New York, N.Y.) 218(4570): 348-53. 1982 Genetic transformation of Drosophila with transposable element vectors Braun, R.E. (1998) Post-transcriptional control of gene expression during spermatogenesis. Seminars in cell and developmental biology 9(4): 483-9. 1998 Post-transcriptional control of gene expression during spermatogenesis Chomczynski, P.; Sacchi, N. (2006) The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on. Nature protocols 1(2): 581-5. 2006 The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on Pittoggi, C. ; Renzi, L. ; Zaccagnini, G. ; Cimini, D. ; Degrassi, F. et al. (1999) A fraction of mouse sperm chromatin is organized in nucleosomal hypersensitive domains enriched in retroposon DNA. Journal of cell science 112(20): 3537-48. 1999 A fraction of mouse sperm chromatin is organized in nucleosomal hypersensitive domains enriched in retroposon DNA Tokuyasu, K. T. ; Peacock, W. J. ; Hardy, R. W. (1972) Dynamics of spermiogenesis in Drosophila melanogaster. I. Individualization process. Zeitschrift fur Zellforschung und mikroskopische Anatomie (Vienna, Austria : 1948) 124(4): 479-506. 1948 Dynamics of spermiogenesis in Drosophila melanogaster. I. Individualization process Stros, M.; Launholt, D.; Grasser, K.D. (2007) The HMG-box: a versatile protein domain occurring in a wide variety of DNA-binding proteins. Cellular and molecular life sciences : CMLS 64(19-20): 2590-606. 2007 The HMG-box: a versatile protein domain occurring in a wide variety of DNA-binding proteins. Cellular and molecular life sciences Cabrero, J.; Palomino-Morales, R.J.; Camacho, J.P.M. (2007) The DNA-repair Ku70 protein is located in the nucleus and tail of elongating spermatids in grasshoppers. Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 15(8): 1093-100. 2007 The DNA-repair Ku70 protein is located in the nucleus and tail of elongating spermatids in grasshoppers. Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Brand, A.H.; Manoukian, A.S.; Perrimon, N. (1994) Ectopic expression in Drosophila. Methods in cell biology 44635-54. 1994 Ectopic expression in Drosophila Xuan, T.; Xin, T.; He, J.; Tan, J.; Gao, Y. et al. (2013) dBre1/dSet1-dependent pathway for histone H3K4 trimethylation has essential roles in controlling germline stem cell maintenance and germ cell differentiation in the Drosophila ovary. Developmental biology 379(2): 167-81. 2013) dBre1/dSet1-dependent pathway for histone H3K4 trimethylation has essential roles in controlling germline stem cell maintenance and germ cell differentiation in the Drosophila ovary Sotolongo, B.; Huang, T.T.F.; Isenberger, E.; Ward, W.S. (2005) An endogenous nuclease in hamster, mouse, and human spermatozoa cleaves DNA into loop-sized fragments. Journal of andrology 26(2): 272-80. 2005 An endogenous nuclease in hamster, mouse, and human spermatozoa cleaves DNA into loop-sized fragments Kim, V. N. ; Han, J. ; Siomi, M. C. (2009) Biogenesis of small RNAs in animals. Nature reviews. Molecular cell biology 10(2): 126-39. 2009 Biogenesis of small RNAs in animals Prakash, L. (1981) Characterization of postreplication repair in Saccharomyces cerevisiae and effects of rad6, rad18, rev3 and rad52 mutations. Molecular and general genetics : MGG 184(3): 471-8. 1981 Characterization of postreplication repair in Saccharomyces cerevisiae and effects of rad6, rad18, rev3 and rad52 mutations. Molecular and general genetics Ripp, C. (2009). Chromatin remodelling during spermiogenesis: characterization of the Drosophila mutant snowballs and analyses of topoisomerase expression during the switch from histones to protamines – Masterarbeit. Entwicklungsbiologie, Philipps-Universität Marburg, Roest, H.P.; van Klaveren, J.; de Wit, J.; van Gurp, C.G.; Koken, M.H. et al. (1996) Inactivation of the HR6B ubiquitin-conjugating DNA repair enzyme in mice causes male sterility associated with chromatin modification. Cell 86(5): 799-810. 1996 Chromatin remodelling during spermiogenesis: characterization of the Drosophila mutant snowballs and analyses of topoisomerase expression during the switch from histones to protamines – Masterarbeit. Entwicklungsbiologie, Philipps-Universität Marburg, Inactivation of the HR6B ubiquitin-conjugating DNA repair enzyme in mice causes male sterility associated with chromatin modification Rathke, C. (2007) Chromatinveränderungen während der Spermienentwicklung von Drosophila melanogaster -von Histonen zu Protaminen -Dissertation. Entwicklungsbiologie, Philipps-Universität Marburg. 2007 Chromatinveränderungen während der Spermienentwicklung von Drosophila melanogaster -von Histonen zu Protaminen -Dissertation Pirotta, V. (1986). Cloning Drosophila genes. Curr. Biol 10, 81-83. 1986 Cloning Drosophila genes Conti, E.; Uy, M.; Leighton, L.; Blobel, G.; Kuriyan, J. (1998) Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin alpha. Cell 94(2): 193-204. 1998 Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin alpha Kaiser, S. (2010) Die Induktion von DNA-Strangbrüchen und deren Reparatur während der Umstrukturierung des Chromatins in der Spermiogenese von Drosophila melanogaster - Masterarbeit. Entwicklungsbiologie, Philipps-Universität Marburg. 2010 Die Induktion von DNA-Strangbrüchen und deren Reparatur während der Umstrukturierung des Chromatins in der Spermiogenese von Drosophila melanogaster Lehman, I.R. (1974) DNA ligase: structure, mechanism, and function. Science (New York, N.Y.) 186(4166): 790-7. 1974 DNA ligase: structure, mechanism, and function Creancier, L.; Morello, D.; Mercier, P.; Prats, A. C. (2000) Fibroblast growth factor 2 internal LITERATUR gene expression during spermatogenesis: identification of regulatory element by transgenic mouse. Biology of reproduction 70(5): 1239-45. 2000 Fibroblast growth factor 2 internal LITERATUR gene expression during spermatogenesis: identification of regulatory element by transgenic mouse Tsuji, L.; Takumi, T.; Imamoto, N.; Yoneda, Y. (1997) Identification of novel homologues of mouse importin alpha, the alpha subunit of the nuclear pore-targeting complex, and their tissue- specific expression. FEBS letters 416(1): 30-4. 1997 Identification of novel homologues of mouse importin alpha, the alpha subunit of the nuclear pore-targeting complex, and their tissuespecific expression Zenkel, M.; Kruse, F.E.; Naumann, G.O.H.; Schlotzer-Schrehardt, U. (2007) Impaired cytoprotective mechanisms in eyes with pseudoexfoliation syndrome/glaucoma. Investigative ophthalmology and visual science 48(12): 5558-66. 2007 Impaired cytoprotective mechanisms in eyes with pseudoexfoliation syndrome/glaucoma Mathe, E.; Bates, H.; Huikeshoven, H.; Deak, P.; Glover, D.M. et al. (2000) Importin-alpha3 is required at multiple stages of Drosophila development and has a role in the completion of oogenesis. LITERATUR (2000) A cell cycle-dependent protein serves as a template-specific translation initiation factor. Genes and development 14(16): 2028-45. 2000 Importin-alpha3 is required at multiple stages of Drosophila development and has a role in the completion of oogenesis. LITERATUR (2000) A cell cycle-dependent protein serves as a template-specific translation initiation factor Abb. 7.4: Mit Hilfe des Hefe-Zwei-Hybrid Systems ist keine Interaktion von Mst77F mit Ocnus und CG12861 nachweisbar. Gezeigt sind Interaktionstests mit Hilfe des Hefe-Zwei-Hybrid Systems. (1) zeigt die Negativkontrolle und (2) die Positivkontrolle. In der Positivkontrolle ist eine Interaktion der zu testeten Proteine durch ein Wachstum und eine Blaufärbung der Kolonien ersichtlich. (3-10) Die ausgestrichenen Kotransformanten weisen keine Blaufärbung der Kolonien auf und zeigen somit keine Interaktion der Mit Hilfe des Hefe-Zwei-Hybrid Systems ist keine Interaktion von Mst77F mit Ocnus und CG12861 nachweisbar Gezeigt sind Interaktionstests mit Hilfe des Hefe-Zwei-Hybrid Systems. (1) zeigt die Negativkontrolle und (2) die Positivkontrolle Sambrook, J.; Fritsch, E.F.; Maniatis, T. (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor Sarcevic, B.; Mawson, A.; Baker, R.T.; Sutherland, R.L. (2002) Regulation of the ubiquitin- conjugating enzyme hHR6A by CDK-mediated phosphorylation. The EMBO journal 21(8): 2009-18. 1989 Molecular cloning: a laboratory manual Regulation of the ubiquitinconjugating enzyme hHR6A by CDK-mediated phosphorylation Saiki, R.K.; Gelfand, D.H.; Stoffel, S.; Scharf, S.J.; Higuchi, R. et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science (New York, N.Y.) 239(4839):487-91. 1988 Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase Lopez-Lastra, M.; Rivas, A.; Barria, M.I. (2005) Protein synthesis in eukaryotes: the growing biological relevance of cap-independent translation initiation. Biological research 38(2-3): 121-46. 2005 Protein synthesis in eukaryotes: the growing biological relevance of cap-independent translation initiation Abb. 7.3: Das Mst77F-Protein zeigt weder Selbstinteraktion noch eine Interaktion mit den Protaminen und Tpl 94D im Hefe-Zwei-Hybrid System. Interaktionstests mit Hilfe des Hefe-Zwei-Hybrid Systems. (1) Negativkontrolle und (2) Positivkontrolle. Bei einer positiven Interaktion sind ein Wachstum und eine Blaufärbung der Kolonien ersichtlich. (3-17) Die ausgestrichenen Transformanten und Kotransformanten zeigen keine Blaufärbung und somit auch keine Interaktion der Fusionsproteine. (3) Mst77F-Proteine zeigen keine Selbstinteraktion und interagiert weder mit (8,9) Protamin A, (12,13) Protamin B, noch mit (16,17) Tpl 94D . Protein zeigt weder Selbstinteraktion noch eine Interaktion mit den Protaminen und Tpl 94D im Hefe-Zwei-Hybrid System. Interaktionstests mit Hilfe des Hefe-Zwei-Hybrid Systems. (1) Negativkontrolle und (2) Positivkontrolle White-Cooper, H.; Schafer, M.A.; Alphey, L.S.; Fuller, M.T. (1998) Transcriptional and post- transcriptional control mechanisms coordinate the onset of spermatid differentiation with meiosis I in LITERATUR Drosophila. Development (Cambridge, England) 125(1): 125-34. 1998 Transcriptional and posttranscriptional control mechanisms coordinate the onset of spermatid differentiation with meiosis I in LITERATUR Drosophila Yanicostas, C. ; Lepesant, J. A. (1990) Transcriptional and translational cis-regulatory sequences of the spermatocyte-specific Drosophila janusB gene are located in the 3' exonic region of the overlapping janusA gene. Molecular and general genetics : MGG 224(3): 450-8. 1990 Transcriptional and translational cis-regulatory sequences of the spermatocyte-specific Drosophila janusB gene are located in the 3' exonic region of the overlapping janusA gene. Molecular and general genetics Spradling, A.C.; Rubin, G.M. (1982) Transposition of cloned P elements into Drosophila germ line chromosomes. Science (New York, N.Y.) 218(4570): 341-7. 1982 Transposition of cloned P elements into Drosophila germ line chromosomes Ulrich, H.D.; Jentsch, S. (2000) Two RING finger proteins mediate cooperation between ubiquitin- conjugating enzymes in DNA repair. The EMBO journal 19(13): 3388-97. 2000 Two RING finger proteins mediate cooperation between ubiquitinconjugating enzymes in DNA repair Chen, H.Y.; Sun, J.M.; Zhang, Y.; Davie, J.R.; Meistrich, M.L. (1998) Ubiquitination of histone H3 in elongating spermatids of rat testes. The Journal of biological chemistry 273(21): 13165-9. 1998 Ubiquitination of histone H3 in elongating spermatids of rat testes Thummel, C. S. ; Boulet, A. M. ; Lipshitz, H. D. (1988) Vectors for Drosophila P-element-mediated transformation and tissue culture transfection. Gene 74(2): 445-56. 1988 Vectors for Drosophila P-element-mediated transformation and tissue culture transfection Braun, R.E. (2001) Packaging paternal chromosomes with protamine. Nature genetics 28(1): 10-2. 2001 Packaging paternal chromosomes with protamine Mason, J.M.; Arndt, K.M. (2004) Coiled coil domains: stability, specificity, and biological implications. Chembiochem : a European journal of chemical biology 5(2): 170-6. 2004 Coiled coil domains: stability, specificity, and biological implications White-Cooper, H. (2010) Molecular mechanisms of gene regulation during Drosophila spermatogenesis. Reproduction (Cambridge, England) 139(1): 11-21. 2010 Molecular mechanisms of gene regulation during Drosophila spermatogenesis Koken, M.H.; Hoogerbrugge, J.W.; Jasper-Dekker, I.; de Wit, J.; Willemsen, R. et al. (1996) Expression of the ubiquitin-conjugating DNA repair enzymes HHR6A and B suggests a role in spermatogenesis and chromatin modification. Developmental biology 173(1): 119-32. 1996 Expression of the ubiquitin-conjugating DNA repair enzymes HHR6A and B suggests a role in spermatogenesis and chromatin modification Cho,C.; Jung-Ha, H.; Willis, W.D.; Goulding, E.H.; Stein, P. et al. (2003) Protamine 2 deficiency leads to sperm DNA damage and embryo death in mice. Biology of reproduction 69(1): 211-7. 2003 Protamine 2 deficiency leads to sperm DNA damage and embryo death in mice Marcon, L.; Boissonneault, G. (2004) Transient DNA strand breaks during mouse and human spermiogenesis new insights in stage specificity and link to chromatin remodeling. Biology of reproduction 70(4): 910-8. 2004 Transient DNA strand breaks during mouse and human spermiogenesis new insights in stage specificity and link to chromatin remodeling Laberge, R.; Boissonneault, G. (2005) On the nature and origin of DNA strand breaks in elongating spermatids. Biology of reproduction 73(2): 289-96. 2005 On the nature and origin of DNA strand breaks in elongating spermatids Shaman, J.A.; Prisztoka, R.; Ward, W.S. (2006) Topoisomerase IIB and an extracellular nuclease interact to digest sperm DNA in an apoptotic-like manner. Biology of reproduction 75(5): 741-8. 2006 Topoisomerase IIB and an extracellular nuclease interact to digest sperm DNA in an apoptotic-like manner Stewart, M. (2007) Molecular mechanism of the nuclear protein import cycle. Nature reviews. LITERATUR Molecular cell biology 8(3): 195-208. 2007 Molecular mechanism of the nuclear protein import cycle Jayaramaiah Raja, S.; Renkawitz-Pohl, R. (2005) Replacement by Drosophila melanogaster protamines and Mst77F of histones during chromatin condensation in late spermatids and role of sesame in the removal of these proteins from the male pronucleus. Molecular and cellular biology 25(14): 6165-77. 2005 Replacement by Drosophila melanogaster protamines and Mst77F of histones during chromatin condensation in late spermatids and role of sesame in the removal of these proteins from the male pronucleus Schupbach, T.; Wieschaus, E. (1991) Female sterile mutations on the second chromosome of Drosophila melanogaster. II. Mutations blocking oogenesis or altering egg morphology. Genetics 129(4): 1119-36. 1991 Female sterile mutations on the second chromosome of Drosophila melanogaster. II. Mutations blocking oogenesis or altering egg morphology James, P.; Halladay, J.; Craig, E.A. (1996) Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast. Genetics 144(4): 1425-36. 1996 Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast Kusano, A.; Staber, C.; Ganetzky, B. (2002) Segregation distortion induced by wild-type RanGAP in Drosophila. Proceedings of the National Academy of Sciences of the United States of America 99(10): 6866-70. 2002 Segregation distortion induced by wild-type RanGAP in Drosophila Williams, R.W.; Rubin, G.M. (2002) ARGONAUTE1 is required for efficient RNA interference in Drosophila embryos. Proceedings of the National Academy of Sciences of the United States of America 99(10): 6889-94. 2002 ARGONAUTE1 is required for efficient RNA interference in Drosophila embryos Lippai, M.; Tirian, L.; Boros, I.; Mihaly, J.; Erdelyi, M. et al. (2000) The Ketel gene encodes a Drosophila homologue of importin-beta. Genetics 156(4): 1889-900. 2000 The Ketel gene encodes a Drosophila homologue of importin-beta Mason, D.A.; Fleming, R.J.; Goldfarb, D.S. (2002) Drosophila melanogaster importin alpha1 and alpha3 can replace importin alpha2 during spermatogenesis but not oogenesis. Genetics 161(1): 157- 70. 2002 Drosophila melanogaster importin alpha1 and alpha3 can replace importin alpha2 during spermatogenesis but not oogenesis Yan, W.; Ma, L.; Burns, K. H.; Matzuk, M.M. (2003) HILS1 is a spermatid-specific linker histone H1-like protein implicated in chromatin remodeling during mammalian spermiogenesis. Proceedings of the National Academy of Sciences of the United States of America 100(18): 10546-51. 2003 HILS1 is a spermatid-specific linker histone H1-like protein implicated in chromatin remodeling during mammalian spermiogenesis Boutanaev, A.M.; Mikhaylova, L.M.; Nurminsky, D.I. (2007) Up-regulation of the Ku heterodimer in Drosophila testicular cyst cells. FEBS letters 581(9): 1707-15. 2007 Up-regulation of the Ku heterodimer in Drosophila testicular cyst cells Kierszenbaum, A.L.; Tres, L.L. (1975) Structural and transcriptional features of the mouse spermatid genome. The Journal of cell biology 65(2): 258-70. 1975 Structural and transcriptional features of the mouse spermatid genome Torok, I.; Strand, D.; Schmitt, R.; Tick, G.; Torok, T. et al. (1995) The overgrown hematopoietic organs-31 tumor suppressor gene of Drosophila encodes an Importin-like protein accumulating in the nucleus at the onset of mitosis. The Journal of cell biology 129(6): 1473-89. 1995 The overgrown hematopoietic organs-31 tumor suppressor gene of Drosophila encodes an Importin-like protein accumulating in the nucleus at the onset of mitosis Markstein, M.; Pitsouli, C.; Villalta, C.; Celniker, S.E.; Perrimon, N. (2008) Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes. Nature genetics 40(4): 476-83. 2008 Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes Jang, S.K.; Krausslich, H.G.; Nicklin, M.J.; Duke, G.M.; Palmenberg, A.C. et al. (1988) A segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. Journal of virology 62(8): 2636-43. 1988 A segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation Temme, C.; Weissbach, R.; Lilie, H.; Wilson, C.; Meinhart, A. et al. (2009) The Drosophila melanogaster Gene cg4930 Encodes a High Affinity Inhibitor for Endonuclease G. The Journal of biological chemistry 284(13): 8337-48. 2009 The Drosophila melanogaster Gene cg4930 Encodes a High Affinity Inhibitor for Endonuclease G Rangan, P.; DeGennaro, M.; Jaime-Bustamante, K.; Coux, R.; Martinho, R.G. et al. (2009) Temporal and spatial control of germ-plasm RNAs. Current biology : CB 19(1): 72-7. 2009 Temporal and spatial control of germ-plasm RNAs Vibranovski, M.D.; Lopes, H.F.; Karr, T.L.; Long, M. (2009) Stage-specific expression profiling of Drosophila spermatogenesis suggests that meiotic sex chromosome inactivation drives genomic relocation of testis-expressed genes. PLoS genetics 5(11): e1000731. 2009 Stage-specific expression profiling of Drosophila spermatogenesis suggests that meiotic sex chromosome inactivation drives genomic relocation of testis-expressed genes Krsticevic, F.J.; Santos, H.L.; Januario, S.; Schrago, C.G.; Carvalho, A.B. (2010) Functional copies of the Mst77F gene on the Y chromosome of Drosophila melanogaster. Genetics 184(1): 295- 307. 2010 Functional copies of the Mst77F gene on the Y chromosome of Drosophila melanogaster Vibranovski, M.D.; Chalopin, D.S.; Lopes, H.F.; Long, M.; Karr, T.L. (2010) Direct evidence for postmeiotic transcription during Drosophila melanogaster spermatogenesis. Genetics 186(1): 431-3. 2010 Direct evidence for postmeiotic transcription during Drosophila melanogaster spermatogenesis White-Cooper, H. ; Davidson, I. (2011) Unique aspects of transcription regulation in male germ cells. Cold Spring Harbor perspectives in biology 3(7): a002626 2011 Unique aspects of transcription regulation in male germ cells Schulze, J.M.; Jackson, J.; Nakanishi, S.; Gardner, J.M.; Hentrich, T. et al. (2009) Linking cell cycle to histone modifications: SBF and H2B monoubiquitination machinery and cell-cycle regulation of H3K79 dimethylation. Molecular cell 35(5): 626-41. 2009 Linking cell cycle to histone modifications: SBF and H2B monoubiquitination machinery and cell-cycle regulation of H3K79 dimethylation Thompson, S.R. (2012) Tricks an IRES uses to enslave ribosomes. Trends in microbiology 20(11): 558-66. 2012 Tricks an IRES uses to enslave ribosomes Yano, R.; Oakes, M.; Yamaghishi, M.; Dodd, J.A.; Nomura, M. (1992) Cloning and characterization of SRP1, a suppressor of temperature-sensitive RNA polymerase I mutations, in Saccharomyces cerevisiae. Molecular and cellular biology 12(12): 5640-51. 1992 Cloning and characterization of SRP1, a suppressor of temperature-sensitive RNA polymerase I mutations, in Saccharomyces cerevisiae Steller, H.; Pirrotta, V. (1986) P transposons controlled by the heat shock promoter. Molecular and cellular biology 6(5): 1640-9. 1986 P transposons controlled by the heat shock promoter St Pierre, S.E.; Ponting, L.; Stefancsik, R.; McQuilton, P. (2014) FlyBase 102--advanced approaches to interrogating FlyBase. Nucleic acids research 42(1): D780-8. 2014 FlyBase 102--advanced approaches to interrogating FlyBase Koken, M.; Reynolds, P.; Bootsma, D.; Hoeijmakers, J.; Prakash, S. et al. (1991B) Dhr6, a Drosophila homolog of the yeast DNA-repair gene RAD6. Proceedings of the National Academy of Sciences of the United States of America 88(9): 3832-6. 1991B Dhr6, a Drosophila homolog of the yeast DNA-repair gene RAD6 Koken, M.H.; Reynolds, P.; Jaspers-Dekker, I.; Prakash, L.; Prakash, S. et al. (1991A) Structural and functional conservation of two human homologs of the yeast DNA repair gene RAD6. Proceedings of the National Academy of Sciences of the United States of America 88(20): 8865-9. 1991A Structural and functional conservation of two human homologs of the yeast DNA repair gene RAD6 Fusionsproteine. Mst77F interagiert im Hefe-Zwei-Hybrid System nicht mit den Proteinen Ocnus und CG12861. LITERATUR Bischof, J.; Maeda, R.K.; Hediger, M.; Karch, F.; Basler, K. (2007) An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases. Proceedings of the National Academy of Sciences of the United States of America 104(9): 3312-7. 2007 An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases Wu, J.; Feng, L.; Hsieh, T. (2010) Drosophila topo IIIalpha is required for the maintenance of mitochondrial genome and male germ-line stem cells. Proceedings of the National Academy of Sciences of the United States of America 107(14): 6228-33. 2010 Drosophila topo IIIalpha is required for the maintenance of mitochondrial genome and male germ-line stem cells Karess, R.E.; Rubin, G.M. (1984) Analysis of P transposable element functions in Drosophila. Cell 38(1): 135-46. 1984 Analysis of P transposable element functions in Drosophila Riparbelli, M.G.; Callaini, G. (2007) The Drosophila parkin homologue is required for normal mitochondrial dynamics during spermiogenesis. Developmental biology 303(1): 108-20. 2007 The Drosophila parkin homologue is required for normal mitochondrial dynamics during spermiogenesis Wouters-Tyrou, D.; Martinage, A.; Chevaillier, P.; Sautiere, P. (1998) Nuclear basic proteins in spermiogenesis. Biochimie 80(2): 117-28. 1998 Nuclear basic proteins in spermiogenesis Chang, T.; Huang, H.; Hsu, J.B.; Weng, S.; Horng, J. et al. (2013) An enhanced computational platform for investigating the roles of regulatory RNA and for identifying functional RNA motifs. BMC bioinformatics 14 Suppl 2S4. 2013 An enhanced computational platform for investigating the roles of regulatory RNA and for identifying functional RNA motifs Kaiser, Sophie Kaiser Sophie Philipps-Universität Marburg ths Prof. Dr. Renkawitz-Pohl Renate Renkawitz-Pohl, Renate (Prof. Dr.)
recordtype opus
id urn:nbn:de:hebis:04-z2014-0377
urn_str urn:nbn:de:hebis:04-z2014-03775
collection Monograph
uri_str http://archiv.ub.uni-marburg.de/diss/z2014/0377
callnumber-raw diss/z2014/0377
callnumber-search diss/z2014/0377
callnumber-sort diss/z2014/0377
callnumber-label diss z2014 0377
callnumber-first diss
callnumber-subject diss z2014
_version_ 1563293957316673536
score 9,205127