Welche Hydroxycinnamoyltransferasen sind in Coleus blumei und in Glechoma hederacea nachweisbar und worin besteht der Einfluss von Ozon auf Melissa officinalis?

Hydroxyzimtsäureester und -amide, z.B. Hydroxycinnamoylchinat, Hydroxycinnamoylshikimat, Hydroxycinnamoyltyramin und Rosmarinsäure, sind im Pflanzenreich sehr weit verbreitet. Pflanzen synthetisieren diese Stoffe zur Abwehr gegen Bakterien oder Pilze, nutzen sie aber auch als UV-Schutz. Die Rosmarin...

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Main Author: Döring, Anne Sarah
Contributors: Petersen, Maike (Prof. Dr.) (Thesis advisor)
Format: Dissertation
Published: Philipps-Universität Marburg 2013
Pharmazeutische Biologie
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140. Anhang Abbildung 144: HPLC-Chromatogramme der Bestimmung von Rosmarinsäure-, Chlorogensäure-und Kaffeesäure-Gehalte in Glechoma hederacea: 71: Standard: Rosmarinsäure, 72: Standard: Chloro- gensäure, 73: Standard: Kaffeesäure, 74: Blatt; 75: Blüte, 76: Stängel, 77: Wurzel. Die Produkt-und Standardpeaks sind mit einem Pfeil markiert. 77

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142. Anhang Abbildung 144: HPLC-Chromatogramme der Bestimmung der RAS-und PAL-Aktivität und der Chlorogensäure-, Rosmarinsäure-und Kaffeesäure-Gehalte in Glechoma hederacea: 78: Nullprobe, 79: Standard: Caf-pHPL, 80: Probe, 81: Nullprobe, 82: Standard: t-Zimtsäure, 83: Probe, 84: Chlorogensäure, 85: Kaffeesäure, 86: Rosmarinsäure, 87: Probe. Die Produkt-und Standardpeaks sind mit einem Pfeil markiert. 86

143. Anhang Abbildung 137: HPLC-Chromatogramme der RAS-Enzymtests nach CbRAS-Expression durch SoluBL21 TM aus 100 ml Bakteriensuspension und His-tag-Aufreinigung. Bestimmung des Aktivitäts- maximums der CbRAS-Elutionsfraktion durch verschiedene Konzentrationen: 36: Standard: 0,5 nmol Caf-pHPL, 37: Elutionsfraktion 1:50 verdünnt (5 min Inkubationszeit), 38: Elutionsfraktion 1:10 verdünnt (5 min Inkubationszeit). Die Produkt-und Standardpeaks sind mit einem Pfeil markiert. 37

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171. Anhang Abbildung 135: HPLC-Chromatogramme der RAS-Enzymtests (Inkubationszeit: 5 min) der Elutions- fraktion des Rohextrakts 1 des CbRAS-Proteins aus inclusion bodies nach der zweiten Solubilisierung mit 1x IB Solubilization Buffer: 29: Standard: 0,5 nmol Caf-pHPL, 30: Elutionsfraktion; 31: Standard:

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173. Abbildung 132: HPLC-Chromatogramme der RAS-Enzymtests (Inkubationszeit: 15 min) nach Resolubisierung und Rückfaltung mit dem " Protein Refolding and Solubilization Kit " der Firma Nova- gen und Aufreinigung über His-tag-Affinitätschromatographie: 10: Standard: 0,5 nmol Caf-pHPL, 11: Elutionsfraktion 1, 12: Elutionsfraktion 2. Die Produkt-und Standardpeaks sind mit einem Pfeil mar- kiert.

174. Anhang Abbildung 134: HPLC-Chromatogramme der RAS-Enzymtests (Inkubationszeit: 5 min) nach erneuter Solubilisierung von Rohextrakt 1 des CbRAS-Proteins aus inclusion bodies: 22: Standard: 0,5 nmol Caf-pHPL, 23: Rohextrakt 1 nach einmonatiger Lagerung bei 4 ºC, 24: zweite Solubilisierung mittels 1x IB Solubilization Buffer, 25: zweite Solubilisierung mittels 10x IB Solubilization Buffer, 26: zweite Solubilisierung mittels 10x IB Solubilization Buffer + 4,5 M Harnstoff, 27: Standard: 0,5 nM Caf-pHPL, 28: zweite Solubilisierung mit 100 mM KH 2 PO 4 + 100 mM L-Arginin. Die Produkt-und Standardpeaks sind mit einem Pfeil markiert. 27 28

175. Anhang Abbildung 133: HPLC-Chromatogramme der RAS-Enzymtests (Inkubationszeit: 5 min) von vier ver- schiedenen Rohextrakten nach Resolubisierung und Rückfaltung mit dem " Protein Refolding and Solubilization Kit " der Firma Novagen: 13: Standard: 0,5 nmol Caf-pHPL, 14: Nullprobe 1, 15: Roh- extrakt 1, 16: Nullprobe 2, 17: Rohextrakt 2, 18: Nullprobe 3, 19: Rohextrakt 3, 20: Nullprobe 4, 21: Rohextrakt 4. Die Produkt-und Standardpeaks sind mit einem Pfeil markiert. 21 22 23

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