RT-PCR Diagnostik von nosokomial übertragbaren Hämorrhagische-Fieber Viren

In dieser Arbeit wurde eine konventionelle RT-PCR für die wichtigsten nosokomial übertragbaren hämorrhagische Fieber auslösenden Viren (Marburg-, Ebola-, Lassa-, Krim-Kongo Hämorrhagisches Fieber Virus) mit einer real-time RT-PCR auf dem Roche LightCycler verglichen. Dabei zeigte sich, dass die konv...

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Bibliographic Details
Main Author: Gebhard, Jos Tilmann Wolf
Contributors: Becker, Stephan (Prof. Dr.) (Thesis advisor)
Format: Dissertation
Language:German
Published: Philipps-Universität Marburg 2013
Hygiene u. Med. Mikrobiologie mit Medizinaluntersuchungsamt
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Table of Contents: Haemorrhagic fevers are caused by a wide range of viruses. Some of them are known to spread easily by person to person contact and are able to cause nosocomial infections with high fatality rates. This applies especially to arenaviruses (Lassa fever and more exceptionally the Junin and Machupo vi-rus), a bunyavirus (Crimean-Congo haemorrhagic fever) and the Filoviridae (Ebola and Marburg viruses). Because these diseases occur initially with non-specific influenza-like symptoms like fever, headache, myalgia etc. clinical dia-gnosis can be difficult. Therefore a quick, sensitive and specific diagnostic me-thod is needed to confirm or rule out infection. Since the viruses are endemic in developing countries the method should also be as cheap as possible. The Re-verse-Transcription PCR combines these characteristics best. In this thesis RT-PCR protocols were developed that were used to detect all viruses mentioned above in one single run (except Ebola-bundibugyo which was discovered after the practical part). An internal control was implemented to minimize the risk of false-negative results.