RT-PCR Diagnostik von nosokomial übertragbaren Hämorrhagische-Fieber Viren

In dieser Arbeit wurde eine konventionelle RT-PCR für die wichtigsten nosokomial übertragbaren hämorrhagische Fieber auslösenden Viren (Marburg-, Ebola-, Lassa-, Krim-Kongo Hämorrhagisches Fieber Virus) mit einer real-time RT-PCR auf dem Roche LightCycler verglichen. Dabei zeigte sich, dass die konv...

Full description

Saved in:
Bibliographic Details
Main Author: Gebhard, Jos Tilmann Wolf
Contributors: Becker, Stephan (Prof. Dr.) (Thesis advisor)
Format: Dissertation
Language:German
Published: Philipps-Universität Marburg 2013
Hygiene u. Med. Mikrobiologie mit Medizinaluntersuchungsamt
Subjects:
Online Access:PDF Full Text
Tags: Add Tag
No Tags, Be the first to tag this record!

1. Linthicum, K.J., Bailey, C.L. Ecology of Crimean–Congo hemorrhagic fever. In: Sonenshine, D.E., Mather, T.N. (Eds.), Ecological Dynamics of Tick-Borne Zoonoses. Oxford University Press, New York, 1994. pp. 392–437.


2. Haferkamp S, Fernando L, Schwarz TF, Feldmann H, Flick R. Intracellular localization of Crimean-Congo Hemorrhagic Fever (CCHF) virus glycoproteins. Virology Journal 2005; 2:42


3. Sanchez AJ, Vincent MJ, Nichol ST. Characterization of the glycoproteins of Crimean-Congo hemorrhagic fever virus. Journal of Virology 2002; 76:7263-75


4. Licata JM, Johnson RF, Han Z, Harty RN. Contribution of ebola virus glycoprotein, nucleoprotein, and VP24 to budding of VP40 virus-like particles. Journal of Virology 2004; 78:7344-51


5. Ebolavirus glycoprotein GP masks both its own epitopes and the presence of cellular surface proteins. J Virol. 2009 Sep;83(18):9596-601. Epub 2009 Jul 8.


6. Lee HW, van der Groen G. Hemorrhagic fever with renal syndrome. Prog Med Virol. 1989;36:62-102. Review.


7. Verduin, Sabine Dittrich, Petra Emmerich, Albert D.M.E. Osterhaus, Jaap T. van Dissel, Roel A. Coutinho Response to Imported Case of Marburg Hemorrhagic Fever, the Netherlands. Emerg Infect Dis. 2009 August; 15(8): 1171–1175. doi: 10.3201/eid1508.090051


8. Vaporciyan AA, Ward PA. The inflammatory response. In Williams Hematology 5. Edition New York: McGraw-Hill; 1995; 48-57


9. Ryabchikova EI, Kolesnikova LV, Netesov SV. Animal pathology of filoviral infections. Curr Top Microbiol Immunol. 1999;235:145-73.


10. Ellis DS, Southee T, Lloyd G, Platt GS, Jones N, Stamford S, Bowen ET, Simpson DI. Congo/Crimean haemorrhagic fever virus from Iraq 1979: I. Morphology in BHK21 cells. Arch Virol. 1981;70(3):189-98.


11. Davies FG, Mwakima F. Qualitative studies of the transmission of Nairobi sheep disease virus by Rhipicephalus appendiculatus.


12. Vieth S, Drosten C, Lenz O, Vincent M, Omilabu S, Hass M, Becker-Ziaja B, Ter Meulen J, Nichol ST, Schmitz H, Günther S. RT-PCR assay for detection of Lassa virus and related Old World arenaviruses targeting the L gene. Transactions of the Royal Society of Tropical Medicine and Hygiene 2007; 12:1253-64


13. Leroy EM, Souquière S, Rouquet P, Drevet D. Re-emergence of ebola haemorrhagic fever in Gabon. Lancet. 2002 Feb 23;359(9307):712


14. Günther S, Weisner B, Roth A, Grewing T, Asper M, Drosten C, Emmerich P, Petersen J, Wilczek M, Schmitz H. Lassa Fever Encephalopathy: Lassa Virus in Cerebrospinal Fluid but not in Serum. J. Infect. Dis. 2001; 184:345-9


15. Haas WH, Breuer T, Pfaff G, Schmitz H, Köhler P, Asper M, Emmerich P, Drosten C, Gölnitz U, Fleischer K, Günther S. Imported Lassa Fever in Germany: Surveillance and Management of Contact Persons. Clin Infect Dis. (2003) 36 (10): 1254-1258.


16. Ergönül O, Celikbaş A, Dokuzoguz B, Eren S, Baykam N, Esener H. Characteristics of patients with Crimean-Congo hemorrhagic fever in a recent outbreak in Turkey and impact of oral ribavirin therapy. Clin Infect Dis. 2004 Jul 15;39(2):284-7. Epub 2004 Jul 2.


17. Leroy EM, Telfer P, Kumulungui B, Yaba P, Rouquet P, Roques P, Gonzalez JP, Ksiazek TG, Rollin PE, Nerrienet E. A serological survey of Ebola virus infection in central African nonhuman primates. J infect Dis. 2004


18. Human infection due to Ebola virus, subtype Côte d'Ivoire: clinical and biologic presentation. J Infect Dis. 1999 Feb;179 Suppl 1:S48-53.


19. Panning M, Laue T, Olschlager S, Eickmann M, Becker S, Raith S, Courbot MC, Nilsson M, Gopal R, Lundkvist A, Caro A, Brown D, Meyer H, Lloyd G, Kummerer BM, Gunther S, Drosten C. Diagnostic reverse-transcription polymerase chain reaction kit for filoviruses based on the strain collections of all European biosafety level 4 laboratories. Journal of Infectious Diseases 2007; 196:199-204


20. The clinical pathology of Crimean-Congo hemorrhagic fever. Reviews of infectious diseases 1989; 4:794-800


21. Fisher-Hoch SP, Platt GS, Neild GH, Southee T, Baskerville A, Raymond RT, Lloyd G, Simpson DI. Pathophysiology of shock and hemorrhage in a fulminating viral infection (Ebola). J. Infect. Dis. 1985 Nov;152:887-94.


22. McCormick JB, King IJ, Webb PA, Johnson KM, O'Sullivan R, Smith ES, Trippel S, Tong TC. A case-control study of the clinical diagnosis and course of Lassa fever. J Infect Dis. 1987 Mar;155(3):445-55.


23. Johnson KM, McCormick JB, Webb PA, Smith ES, Elliott LH, and King IJ. Clinical virology of Lassa fever in hospitalized patients. J. Infect. Dis. 1987 155: 456-464


24. Peters CJ, Zaki SR. Role of the endothelium in viral hemorrhagic fevers. Crit Care Med. 2002 May;30(5 Suppl):S268-73. Review.


25. Price ME, Fisher-Hoch SP, Craven RB, McCormick JB. A prospective study of maternal and fetal outcome in acute Lassa fever infection during pregnancy. BMJ. 1988 Sep 3;297(6648):584-7.


26. Joubert JR, King JB, Rossouw DJ, Cooper R. A nosocomial outbreak of Crimean-Congo haemorrhagic fever at Tygerberg Hospital. Part III. Clinical pathology and pathogenesis. S Afr Med J. 1985 Nov 9;68(10):722-8.


27. Ryabchikova E, Kolesnikova L, Smolina M, Tkachev V, Pereboeva L, Baranova S, Grazhdantseva A, Rassadkin Y. Ebola virus infection in guinea pigs: presumable role of granulomatous inflammation in pathogenesis. Arch Virol. 1996;141(5):909-21.


28. Fisher-Hoch SP. Acute sensorineural deafness in Lassa fever. JAMA. 1990


29. McCormick JB, Webb PA, Krebs JW, Johnson KM, Smith ES., A prospective study of the epidemiology and ecology of Lassa Fever. J infect Dis. 1987 Mar; 155(3): 437-44


30. Schmaljohn CS, Nichol ST. Bunyaviridae in Fields Virology 5 th Edition 2007; 1741-1789. ISBN-10: 0781760607


31. Case-control study of Mastomys natalensis and humans in Lassa virus-infected households in Sierra Leone. Am J Trop Med Hyg 1983;32: 829-37


32. Sanchez A, Rollin PE. Complete genome sequence of an Ebola virus (Sudan species) responsible for a 2000 outbreak of human disease in Uganda. Virus Res. 2005 Oct;113(1):16-25.


33. Flick R, Whitehouse CA. Crimean-Congo Hemorrhagic Fever Virus. Current Molecular Medicine 2005; 5:753-60


34. Leroy EM, Baize S, Lu CY, et al. Diagnosis of Ebola haemorrhagic fever by RT-PCR in an epidemic setting. J Med Virol 2000 Apr;60(4):463-7


35. Gonzalez JP, Nakoune E, Slenczka W, Vidal P, Morvan JM. Ebola and Marburg virus antibody prevalence in selected populations of the Central African Republic. Microbes and infection 2000 2:39-44


36. Harcourt BH, Sanchez A, Offermann MK. Ebola virus selectively inhibits responses to interferons, but not to interleukin-1beta, in endothelial cells. Journal of Virology 1999; 73:3491-6


37. McCormick JB, King IJ, Webb PA, Scribner CL, Craven RB, Johnson KM, Elliott LH, Belmont-Williams R. Lassa fever. Effective therapy with ribavirin. N Engl J Med. 1986 Jan 2;314(1):20-6.


38. Mayer CF. Epidemic hemorrhagic fever of the Far East or endemic hemorrhagic nephroso-nephritis; morphology and pathogenesis. Lab Invest. 1952;1(3):291- 311.


39. Shepherd AJ, Swanepoel R, Cornel AJ, Mathee O. Experimental studies on the replication and transmission of Crimean-Congo hemorrhagic fever virus in some African tick species. Am J Trop Med Hyg. 1989 Mar;40(3):326-31.


40. Peters CJ, Sanchez A, Rollin PE, Ksiazek TG, Murphy FA. Filoviridae: Marburg and Ebola Viruses. Fields Virology, Third Edition 1996; 1161-76; ISBN: 0-7817-0253-4


41. Jasenosky LD, Kawaoka Y. Filovirus budding. Virus Res. 2004


42. Leroy EM, Kumulungui B, Pourrut X, Rouquet P, Hassanin A, Yaba P, Delicat A, Paweska JT, Gonzalez JP, Swanepoel R. Fruit bats as reservoirs of Ebola virus. Nature 2005 Dec 1; 438:575-6


43. Volchkov VE, Becker S, Volchkova VA, Ternovoj VA, Kotov AN, Netesov SV, Klenk HD. GP mRNA of Ebola virus is edited by the Ebola virus polymerase and by T7 and vaccinia virus polymerases. Virology. 1995 Dec 20;214(2):421-30.


44. Ter Meulen J, Lukashevich I, Sidibe K, Inapogui A, Marx M, Dorlemann A, et al. Hunting of peridomestic rodents and consumption of their meat as possible risk factors for rodent-to-human transmission of Lassa virus in the Republic of Guinea. Am J Trop Med Hyg 1996;55; 661-6.


45. Identification of the Ebola virus glycoprotein as the main viral determinant of vascular cell cytotoxicity and injury. Nature Medicine 2000; 6:886-9


46. Holodniy M, Kim S, Katzenstein D, Konrad M, Groves E, Merigan TC. Inhibition of human immunodeficiency virus gene amplification by heparin. J Clin Microbiol. 1991 Apr;29(4):676-9.


47. Johnson KM, Lange JV, Webb PA, Murphy FA. Isolation and partial characterisation of a new virus causing acute haemorrhagic fever in Zaire. Lancet. 1977 Mar 12;1(8011):569-71.


48. Grolla A, Lucht A, Dick D, Strong JE, Feldmann H. Laboratory diagnosis of Ebola and Marburg hemorrhagic fever. Bull Soc Pathol Exot. 2005


49. Frame JD, Baldwin JM Jr, Gocke DJ, Troup JM. Lassa fever, a new virus disease of man from West Africa. I. Clinical description and pathological findings. Am J Trop Med Hyg. 1970 Jul;19(4):670-6.


50. Richmond JK, Baglole DJ. Lassa fever: Epidemiology, clinical features, and social consequences. BMJ 2003; 327: 1271-75


51. Lukashevich IS, Clegg JC, Sidibe K. Lassa virus activity in Guinea: distribution of human antiviral antibody defined using enzyme-linked immunosorbent assay with recombinant antigen. J Med Virol. 1993


52. Lenz O. Lassa Virus Z Protein Is a Matrix Protein Sufficient for the Release of Virus-Like Particles. Journal of Virology 2003; 10: 10700-10705


53. Feldmann H, Mühlberger E, Randolf A, Will C, Kiley MP, Sanchez A, Klenk HD. Marburg virus, a filovirus: messenger RNAs, gene order, and regulatory elements of the replication cycle. Virus Res. 1992 Jun;24(1):1-19.


54. Towner JS, Pourrut X, Albariño CG, Nkogue CN, Bird BH, Grard G, Ksiazek TG, Gonzalez JP, Nichol ST, Leroy EM. Marburg virus infection detected in a common African bat. PLoS One. 2007 Aug 22;2(1):e764.


55. Villinger F, Rollin PE, Brar SS, Chikkala NF, Winter J, Sundstrom JB, Zaki SR, Swanepoel R, Ansari AA, Peters CJ. Markedly elevated levels of interferon (IFN)-gamma, IFN-alpha, interleukin (IL)-2, IL-10, and tumor necrosis factor-alpha associated with fatal Ebola virus infection. J Infect Dis. 1999


56. Drosten C, Kummerer BM, Schmitz H, et al. Molecular diagnostics of viral hemorrhagic fevers. Antiviral Res 2003 Jan;57(1-2):61-87


57. Hartlieb B, Modrof J, Mühlberger E, Klenk HD, Becker S. Oligomerization of Ebola virus VP30 is essential for viral transcription and can be inhibited by a synthetic peptide. Journal of biological chemistry 2003; 278:41830-6


58. Hayes CG, Burans JP, Ksiazek TG, Del Rosario RA, Miranda ME, Manaloto CR, Barrientos AB, Robles CG, Dayrit MM, Peters CJ. Outbreak of fatal illness among captive macaques in the Philippines caused by an Ebola-related filovirus. Am J Trop Med Hyg. 1992 Jun;46(6):664-71.


59. Drosten C, Gottig S, Schilling S, Asper M, Panning M, Schmitz H, Gunther S. Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR. Journal of clinical microbiology 2002; 40(7):2323-30


60. Volchkov VE, Volchkova VA, Muhlberger E, Kolesnikova LV, Weik M, Dolnik O, Klenk HD. Recovery of infectious Ebola virus from complementary DNA: RNA editing of the GP gene and viral cytotoxicity. Science. 2001 Mar 9;291(5510):1965-9. Epub 2001 Feb 1


61. Sanchez A, Kiley MP, Holloway BP, Auperin DD. Sequence analysis of the Ebola virus genome: organization, genetic elements, and comparison with the genome of Marburg virus. Virus Res. 1993 Sep;29(3):215-40


62. Downs WG, Anderson CR, Spence L, Aitken T, Greenhal AH. Tacaribe Virus, a New Agent Isolated from Artibeus Bats and Mosquitoes in Trinidad, West Indies. Am. J. Trop. Med. Hyg., 12(4), 1963, pp. 640-646


63. Dominguez-Soto A, Aragoneses-Fenoll L, Martin-Gayo E, Martinez-Prats L, Colmenares M, Naranjo-Gomez M, Borras FE, Munoz P, Zubiaur M, Toribio ML, Delgado R, Corbi AL. The DC-SIGN-related lectin LSECtin mediates antigen capture and pathogen binding by human myeloid cells. Blood. 2007 Jun 15;109(12):5337-45. Epub 2007 Mar 5.


64. Hoogstraal, H. The epidemiology of tick-borne Crimean–Congo hemorrhagic fever in Asia, Europe, and Africa. J. Med. Entomol. 1979. 15, 307–417.


65. Geisbert TW, Hensley LE, Jahrling PB, Larsen T, Geisbert JB, Paragas J, Young HA, Fredeking TM, Rote WE, Vlasuk GP. Treatment of Ebola virus infection with a recombinant inhibitor of factor VIIa/tissue factor: a study in rhesus monkeys. Lancet 2003; 362:1953-8


66. Tomori O, Fabiyi A, Sorungbe A, Smith A, McCormick JB. Viral hemorrhagic fever antibodies in Nigerian populations. Am J Trop Med Hyg. 1988


67. Ksiazek TG. Clinical, virologic, and immunologic follow-up of convalescent Ebola hemorrhagic fever patients and their household contacts, Kikwit, Democratic Republic of the Congo. Commission de Lutte contre les Epidémies à Kikwit. J Infect Dis. 1999 Feb;179 Suppl 1:S28-35.


68. Virus-like particles exhibit potential as a pan-filovirus vaccine for both Ebola and Marburg viral infections. Vaccine 2005; 23:3033-42


69. Jones SM, Feldmann H, Stroher U, Geisbert JB, Fernando L, Grolla A, Klenk HD, Sullivan NJ, Volchkov VE, Fritz EA, Daddario KM, Hensley LE, Jahrling PB, Geisbert TW. Live attenuated recombinant vaccine protects nonhuman primates against Ebola and Marburg viruses. Nature Medicine 2005; 11:786-90


70. Kolesnikova L, Mühlberger E, Ryabchikova E, Becker S. Ultrastructural organization of recombinant Marburg virus nucleoprotein: comparison with Marburg virus inclusions. J Virol. 2000 Apr;74(8):3899-904.


71. Ito H, Watanabe S, Takada A, Kawaoka Y. Ebola virus glycoprotein: proteolytic processing, acylation, cell tropism, and detection of neutralizing antibodies. J Virol. 2001 Feb;75(3):1576-80.


72. Infection and activation of monocytes by Marburg and Ebola viruses. J Virol. 2001 Nov;75(22):11025-33.


73. Han Z, Boshra H, Sunyer JO, Zwiers SH, Paragas J, Harty RN. Biochemical and functional characterization of the Ebola virus VP24 protein: implications for a role in virus assembly and budding. Journal of Virology 2003; 77:1793-800


74. Saijo M, Niikura M, Ikegami T, et al. Laboratory diagnostic systems for Ebola and Marburg hemorrhagic fevers developed with recombinant proteins. Clin Vaccine Immunol 2006 Apr;13(4):444-51


75. Shimojima M, Takada A, Ebihara H, Neumann G, Fujioka K, Irimura T, Jones S, Feldmann H, Kawaoka Y. Tyro3 family-mediated cell entry of Ebola and Marburg viruses. J Virol. 2006 Oct;80(20):10109-16.


76. Geisbert TW, Hensley LE, Larsen T, Young HA, Reed DS, Geisbert JB, Scott DP, Kagan E, Jahrling PB, Davis KJ. Pathogenesis of Ebola hemorrhagic fever in cynomolgus macaques: evidence that dendritic cells are early and sustained targets of infection. Am J Pathol. 2003 Dec;163(6):2347-70.


77. Rowe AK, Bertolli J, Khan AS, Mukunu R, Muyembe-Tamfum JJ, Bressler D, Williams AJ, Peters CJ, Rodriguez L, Feldmann H, Nichol ST, Rollin PE, Saijo M, Georges-Courbot MC, Marianneau P, et al. Development of recombinant nucleoprotein-based diagnostic systems for Lassa fever. Clin Vaccine Immunol 2007 Sep;14(9):1182-9


78. Volchkov VE, Feldmann H, Volchkova VA, Klenk HD. Processing of the Ebola virus glycoprotein by the proprotein convertase furin. Proc Natl Acad Sci U S A. 1998 May 12;95(10):5762-7


79. Sullivan N, Yang ZY, Nabel GJ. Ebola virus pathogenesis: implications for vaccines and therapies. Journal of Virology 2003; 77:9733-7


80. Monteiro L, Bonnemaison D, Vekris A, Petry KG, Bonnet J, Vidal R, Cabrita J, Mégraud F. Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model. J Clin Microbiol. 1997 Apr;35(4):995-8.


81. Phosphoinositide-3 kinase-Akt pathway controls cellular entry of Ebola virus.


82. Towner JS, Sealy TK, Khristova ML, Albariño CG, Conlan S, Reeder SA, Quan PL, Lipkin WI, Downing R, Tappero JW, Okware S, Lutwama J, Bakamutumaho B, Kayiwa J, Comer JA, Rollin PE, Ksiazek TG, Nichol ST. Newly discovered ebola virus associated with hemorrhagic fever outbreak in Uganda. PLoS Pathog. 2008 Nov;4(11): e1000212. Epub 2008 Nov 21.


83. Demby AH, Chamberlain J, Brown DW, Clegg CS. Early diagnosis of Lassa fever by reverse transcription-PCR. J Clin Microbiol. 1994 Dec; 32(12):2898- 903


84. Seesod N, Lunderberg J, Hedrum A, Aslund L, Holder A, Thaithong S, Uhlén M. Immunomagnetic purification to facilitate DNA diagnosis of Plasmodium falciparum. J Clin Microbiol. 1993 Oct;31(10):2715-9.


85. Shepherd AJ, Swanepoel R, Leman PA, Shepherd SP. Comparison of methods for isolation and titration of Crimean-Congo hemorrhagic fever virus. J Clin Microbiol. 1986 Oct;24(4):654-6.


86. Mitchell SW, McCormick JB. Physicochemical inactivation of Lassa, Ebola, and Marburg viruses and effect on clinical laboratory analyses. J Clin Microbiol. 1984 Sep;20(3):486-9.


87. Elliott LH, McCormick JB, Johnson KM. Inactivation of Lassa, Marburg, and Ebola viruses by gamma irradiation. J Clin Microbiol. 1982 October; 16(4): 704– 708.


88. Krähling V, Dolnik O, Kolesnikova L, Schmidt-Chanasit J, Jordan I, Sandig V, Günther S, Becker S. Establishment of fruit bat cells (Rousettus aegyptiacus) as a model system for the investigation of filoviral infection. PLoS Negl Trop Dis. 2010 Aug 24;4(8):e802.


89. Karti SS, Odabasi Z, Korten V, Yilmaz M, Sonmez M, Caylan R, Akdogan E, Eren N, Koksal I, Ovali E, Erickson BR, Vincent MJ, Nichol ST, Comer JA, Rollin PE, Ksiazek TG. Crimean-Congo hemorrhagic fever in Turkey. Emerging infectious diseases 2004; 10:1379-84


90. Towner JS, Rollin PE, Bausch DG, et al. Rapid diagnosis of Ebola hemorrhagic fever by reverse transcription-PCR in an outbreak setting and assessment of patient viral load as a predictor of outcome. J Virol 2004


91. Sanchez A, Trappier SG, Mahy BW, Peters CJ, Nichol ST. The virion glycoproteins of Ebola viruses are encoded in two reading frames and are expressed through transcriptional editing. Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3602-7


92. Hoenen T, Volchkov V, Kolesnikova L, Mittler E, Timmins J, Ottmann M, Reynard O, Becker S, Weissenhorn W. VP40 octamers are essential for Ebola virus replication. Journal of Virology 2005; 79:1898-905


93. Gibb TR, Norwood DA Jr, Woollen N, et al. Development and evaluation of a fluorogenic 5' nuclease assay to detect and differentiate between Ebola virus subtypes Zaire and Sudan. J Clin Microbiol 2001 Nov;39(11):4125-30


94. Jahrling PB, Geisbert TW, Dalgard DW, Johnson ED, Ksiazek TG, Hall WC, Peters CJ. Preliminary report: isolation of Ebola virus from monkeys imported to USA. Lancet. 1990 Mar 3;335(8688):502-5.


95. Weidmann M, Muhlberger E, Hufert FT. Rapid detection protocol for filoviruses. J Clin Virol 2004 May;30(1):94-9