Crosslinking studies of the bacterial RNase P holoenzyme and functional characterisation of the novel protein-only RNase P from Arabidopsis thaliana
Ribonuclease P (RNase P) is the ubiquitous processing enzyme that generates the mature 5'-termini of tRNA molecules. In Bacteria and Archaea the enzyme is a ribonucleoprotein, whereas in some Eukarya the RNAse P is exclusively proteinaceous. This work is focused on two topics: (i) crosslinking...
Shranjeno v:
Glavni avtor: | |
---|---|
Drugi avtorji: | |
Format: | Dissertation |
Jezik: | angleščina |
Izdano: |
Philipps-Universität Marburg
2012
|
Teme: | |
Online dostop: | PDF-Volltext |
Oznake: |
Označite
Brez oznak, prvi označite!
|
Izvleček: | Ribonuclease P (RNase P) is the ubiquitous processing enzyme that generates the mature 5'-termini of tRNA molecules. In Bacteria and Archaea the enzyme is a ribonucleoprotein, whereas in some Eukarya the RNAse P is exclusively proteinaceous. This work is focused on two topics: (i) crosslinking studies of bacterial RNase P holoenzyme utilizing novel 2’- ribooligonucleotides and (ii) functional investigations to the catalytical mechanism of recently discovered protein-only RNase P from Arabidopsis thaliana. |
---|---|
DOI: | 10.17192/z2012.0778 |