Biodegradable multifunctional nanocarriers for pDNA and siRNA delivery

In this thesis, biodegradable non-viral polymeric nucleic acids delivery vectors were characterized concerning biophysicochemical parameters. In the first part of this research, to answer the questions: why the principle of DNA transfection cannot be directly applied for siRNA transfection, we inv...

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Bibliographic Details
Main Author: Zheng, Mengyao
Contributors: Kissel, Thomas (Prof. Dr.) (Thesis advisor)
Format: Dissertation
Language:English
Published: Philipps-Universität Marburg 2012
Pharmazeutische Technologie und Biopharmazie
Subjects:
DNA
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25. Abschnitt der Pharmazeutischen Prüfung Sep. 2010


26. In Kapitel 7 wurden neuartige bioabbaubare und biokompatible Polymere der Zusamensetzung Poly (PEG-co-(BMDO-co-DMAEMA) für die Gen-Transfektion erfolgreich bezüglich ihrer physikalisch-chemischen und in vitro biologischen Eigenschaften charakterisiert. Die quarternierten Copolymere zeigten niedrige Zytotoxizitä t als die quarternisierten Copolymere sowie positive Ergebnisse in der DNA-Transfektion in vitro.


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29. In Kapitel 6 wurden hyperflexible Triazin-Dendrimere der Generationen 2-4 als neuartige siRNA-Delivery-Systeme bezüglich ihrer physikalisch-chemischer und biologischer in vitro und in vivo Eigenschaften untersucht und diskutiert. In diesem Kapitel wurden die thermodynamische Eigenschaften durch molekulare Modellierung simuliert, und der Einfluss der Flexibilitä t wurde systematisch untersucht und diskutiert.


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39. In Kapitel 3 haben wir anhand der Untersuchung des Einflusses der Kupplungsdichten von PCL-b-PEG-Ketten auf physikalisch-chemische Eigenschaften, DNA-Komplexierung und Transfektionseffizienz gefunden, dass der " endosomal escape " eine sehr wichtige Rolle für die


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44. Abitur, Gymnasium, Beijing, China (Note: sehr gut)


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