Analyse von phenolischen Naturstoffen

Vorkommen von Rosmarinsäure und Chlorogensäure im Pflanzenreich: Rosmarinsäure (RA) und Chlorogensäure (CA) sind Kaffeesäureester. Sie sind im Pflanzenreich weit verbreitet, vermutlich als Abwehrstoffe. In der vorliegenden Studie wurden mehr als 240 Pflanzenarten zum Nachweis von Rosmarinsäure un...

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1. Verfasser: Abdullah, Yana Ghassan
Beteiligte: Petersen, Maike (Prof.) (BetreuerIn (Doktorarbeit))
Format: Dissertation
Sprache:Deutsch
Veröffentlicht: Philipps-Universität Marburg 2010
Pharmazeutische Biologie
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7. Abb. 1: Extrakte aus: CA – CA – Theobroma cacao – Lantana camara – Eryngium bourgatii – Abutilon theophrastis -Asclepias syriaca -Nemophila menziesii – Gentiana asclepiadea -RA -Gentiana lutea - Acanthus longifolius – Phlomis tuberosa -Verbena officinalis -Hydrophyllum virginicum – Plectranthus ciliatus -Maranta leuconeura.


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10. Zur Analyse von RA und CA diente als Laufmittel Ethylacetat/Chloro- form/Ameisensäure 50:40:10. Danach wurde die Platte mit Naturstoffreagenz A und PEG 400 besprüht. Zur Detektion von Rutin wurde das Laufmittel Ethylacetat/Was- ser/Ameisensäure 32,5:10:7,5 benutzt, und die Platten mit Naturstoffreagenz A besprüht.


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12. Dünnschichtchromatografische Analysen In den folgenden Abbildungen sind die dünnschichtchromatografischen Analysen der Blattextrakte dargestellt, in denen über HPLC RA, CA oder Rutin identifiziert worden waren. Diese DC-Analysen dienten der Verifizierung des Vorkommens der jeweiligen Naturstoffe. Nur wenn beide Analysen (HPLC und DC) positiv ausfielen, wurde die Substanz als RA, CA oder Rutin angegeben.


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24. Abb. 19: Extrakte aus: Anthriscus cerefolium -Peucedanum officinale -Levisticum officinale -RA -CA -Seseli libanotis -Seseli hippomarathrum -Dorema ammoniacum -Apium graveolens.


25. Abb. 10: Extrakte aus: Aralia california -Viburnum hupehense -Viburnum dilatatum – RA – CA - Viburnum lantanta -Geranium sanguineum -Geranium swatense – Phuopsis stylosa.


26. Abb. 15: Extrakte aus: Arnica chamissonis -Abroma augustum -Tagetes tenuifolia -Achillea millefolia -RA -CA -Gaura biennis -Oenothera missouriensis – Lopezia racemosa.


27. Abb. 24: Extrakte aus: Ataenidia conferta -Calathea warscewiczii -Ctenanthe setosa -Maranta arundinacea -Rutin -Maranta depressa.


28. Abb. 4: Extrakte aus: CA -Blechnum arcuatum -Blechnum occidentale – Helicteres jamaicensis – Salvia officinalis – CA – RA -Helicteres jamaicensis – Dombeya rotundifolia -Blechnum gibbum - Blechnum brasiliense -Hydrophyllum canadense -Blechnum occidentale.


29. Abb. 2: Extrakte aus: CA -Knautia dipsacifolia – Catharanthus roseus -Rubia tinctorum -Geranium sylvaticum -RA.


30. Abb. 8: Extrakte aus: CA -Satureja montana – Ligusticum scoticum – RA – CA -Foeniculum vulgare - Carum carvi -Seseli libanotis -Cephalaria gigantia.


31. Abb. 7: Extrakte aus: CA -Verbena spec. – Scrophularia nodosa -Leonurus cardiaca -Seseli hippomarathrum -Satureja montana – RA -CA -Ilex aquifolium -Stromanthe amabilis -Glechoma hederacea -Dracocephalum spec. -Galium boreale.


32. Abb. 3: Extrakte aus: Centaurea macrocephala -Tilia platyphyllos -Verbena officinalis -Verbena urticifolia -Eleutherococcus senticosus – CA -Anthriscus sylvestris.


33. Abb. 21: Extrakte aus: Cerinthe major -Calamintha nepeta -Mentha aquatica -Origanum majorana - Lavandula multifida -Lavandula angustifolia -RA – CA -Maranta leuconeura -Maranta depressa - Thalia geniculata -Canna indica -Canna edulis -Salvia officinalis.


34. Abb. 25: Extrakte aus: Ctenananthe kummeriana -Rutin -Thalia dealbata -Calathea undulata – Orchidantha maxillarioides.


35. Abb. 23: Extrakte aus: Ctenanthe burle marxii -Calathea media -Rutin -Calthea variegata – Pleiostachya pruinosa – Strelitzia reginae -Calathea rotundifolia -Rutin.


36. Abb. 13: Extrakte aus: Helianthus annuus – Philadephus spec. -Cerinthe major – Buglossoides arvensis – CA – RA -Lindeloflia longiflora – Cichorium intybus – Heliotropium amplexicaule.


37. Abb. 6: Extrakte aus: Jacobinia zelandia -Lythrum alatum -Juncus effusus – Saracha edulis -Salvia splendes – Linaria triornithophora – Collinsonia canadensis – Melissa officinalis – CA – RA - Hydrophyllum virginicum – Dracocephalum spec. – Cenolophium denudatum -Galium rubioides – Micromeria thymifolia – Origanum majorana -Digitalis lanata – Digitalis lutea -Plantago schwarzenbergiana.


38. Abb. 20: Extrakte aus: Lavandula angustifolia -Viburnum lantanta -Atropa belladonna -CA -RA - Astrantia major -Carum carvi -Foeniculum vulgare -Ligusticum scoticum -Lavandula multifida.


39. Abb. 11: Extrakte aus: Lonicera ferdinandii -Lonicera demissa -Diervilla trifolia -Diervilla lonicera – CA – RA -Chionodoxa luciliae – Cucurbita spec. – Cephalaria gigantia.


40. Abb. 9: Extrakte aus: Lonicera kamtschatica -Lonicera emphyllocalyx -Lonicera demissa -Lonicera ferdinandii – CA – RA -Lonicera syringantha – Asclepias syriaca – Erodium manescavii.


41. Abb. 18: Extrakte aus: Physalis alkekengi -Salvia splendens – Lycopersicon esculentum – RA – CA - Cucurbita spec. – Cephalaria gigantia – Convolvulus tricolor – Aralia california.


42. Abb. 16: Extrakte aus: RA – CA -Plantago nivalis – Plantago media -Plantago schwarzenbergiana – Plantago sempervirens -Plantago media-Penstemon hirsuta -Penstemon serrulatus -Penstemon digitalis.


43. Abb. 26: Extrakte aus: Rutin -Canna indica -Canna edulis.


44. Abb.12: Extrakte aus: Scabiosa atropurpurea -Scabiosa caucasia -Dombeya rotundifolia -CA.


45. Abb. 22: Extrakte aus: Stromanthe sanguinea -Rutin -Calathea lancifolia -Maranta noctiflora - Ctenananthe lubbersiana -Maranta leuconeura -Calathea insignis.


46. Abb. 14: Extrakte aus: Succisella inflexa -Hedera colchica -Hedera helix – Juncus effusus – RA – CA -Dipsacus laciniatus – Symphytum officinale – Echium italicum.


47. Abb. 5: Extrakte aus: Tilia japonica -Tilia amurensis -Tilia americana – CA -Tilia tomentosa -Tilia cordata.


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